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Genotoxic effects of heavy metal cadmium on growth, biochemical, cyto-physiological parameters and detection of DNA polymorphism by RAPD in Capsicum annuum L. : an important spice crop of India
Joint Authors
Islam, Rumana
Ansari, M. Y. K.
Choudhary, Sana
Bhat, Towseef Muhsin
Jahan, Nusrat
Source
Saudi Journal of Biological Sciences
Issue
Vol. 21, Issue 5 (30 Nov. 2014), pp.465-472, 8 p.
Publisher
Publication Date
2014-11-30
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Topics
Abstract EN
Abstract The present study was designed to investigate the effects of cadmium (Cd) on biochemical, physiological and cytological parameters of Capsicum annuum L.
treated with five different concentrations (20, 40, 60, 80 and 100 ppm) of the metal.
Shoot–root length, pigment and protein content showed a continuous decrease with increasing Cd concentrations and the maximal decline was observed at the higher concentration.
Proline content was found to be increased upto 60 ppm plants selected at mild doses which resemble control at the DNA level.
This investigation showed that RAPD marker is a useful tool for evaluation of genetic diversity and relationship among different metal concentrations.
while at higher concentrations it gradually decreased.
MDA content and chromosomal aberrations increased as the concentration increased.
Additionally Random amplified polymorphic DNA (RAPD) technique was used for the detection of genotoxicity induced by Cd.
A total of 184 bands (62 polymorphic and 122 monomorphic) were generated in 5 different concentrations with 10 primers where primer OPA-02 generated the highest percentage of polymorphism (52.63%).
Dendrogram showed that control, R1 and R2 showed similar cluster and R4 and R5 grouped with R3 into one cluster, which showed that plants from higher doses showed much difference than the
American Psychological Association (APA)
Islam, Rumana& Ansari, M. Y. K.& Choudhary, Sana& Bhat, Towseef Muhsin& Jahan, Nusrat. 2014. Genotoxic effects of heavy metal cadmium on growth, biochemical, cyto-physiological parameters and detection of DNA polymorphism by RAPD in Capsicum annuum L. : an important spice crop of India. Saudi Journal of Biological Sciences،Vol. 21, no. 5, pp.465-472.
https://search.emarefa.net/detail/BIM-410056
Modern Language Association (MLA)
Islam, Rumana…[et al.]. Genotoxic effects of heavy metal cadmium on growth, biochemical, cyto-physiological parameters and detection of DNA polymorphism by RAPD in Capsicum annuum L. : an important spice crop of India. Saudi Journal of Biological Sciences Vol. 21, no. 5 (Nov. 2014), pp.465-472.
https://search.emarefa.net/detail/BIM-410056
American Medical Association (AMA)
Islam, Rumana& Ansari, M. Y. K.& Choudhary, Sana& Bhat, Towseef Muhsin& Jahan, Nusrat. Genotoxic effects of heavy metal cadmium on growth, biochemical, cyto-physiological parameters and detection of DNA polymorphism by RAPD in Capsicum annuum L. : an important spice crop of India. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 5, pp.465-472.
https://search.emarefa.net/detail/BIM-410056
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 471-472
Record ID
BIM-410056