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Vancomycin resistance among methicillin resistant staphylococcus aureus isolates from general hospitals
Joint Authors
Ayyub, Nidal S.
Abd al-Hamid, Rashad A.
Source
al-Kindy College Medical Journal
Issue
Vol. 10, Issue 1 (30 Jun. 2014), pp.80-84, 5 p.
Publisher
University of Baghdad al-Kindi College of Medicine
Publication Date
2014-06-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Abstract EN
Background : Multidrug resistant methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial and community acquired infections.
The glycopeptides vancomycin has been proposed as the drug of choice for treating such infections; this lead to the emergence of vancomycin intermediate sensitive S.
aureus (VISA) and vancomycin resistant S.aureus (VRSA).
Objectives : To identify the vancomycin resistance both phenotypically and genotypically among MRSA isolates from different hospitals and to determine the sensitivity of these isolates to different antimicrobial agents.
Methods : A total of 204 S.
aureus isolates were obtained randomly from various clinical specimens including (wound swab, burn swab, ear swab, urine, sputum, blood and other body fluids) from different inpatient and outpatient who were attending different hospitals in Baghdad.
The susceptibility pattern of the S.
aureus isolates to different antibiotics was determined by disk diffusion method and vancomycin minimum inhibitory concentration (MIC) for MRSA isolates were determined using broth dilution method following clinical laboratory standard institution (CLSI) guidelines.
Van A gene was amplified by PCR using standard primers.
Results : All VRSA isolates were MRSA.
Twelve VRSA isolates were positive for van A gene, while the remaining ten isolates were negative.
All VRSA had a vancomycin MIC of 16μg/ml or more.
In the present study, VRSA showed resistance to a wide range of antimicrobial agents (Ampicillin, Cefalothin, Cefoxitin, Erythromycin, Gentamycin, Oxacillin, Penicillin, Rifampin, Tetracycline and Trimethoprim).
Conclusions : There were high incidences of resistance to the commonly used antibiotics among VRSA isolates compared to VISA and VSSA.
Further molecular studies such as PCR technique to identify genes rather than van A (e.
g van HAX analogue) might be suitable to predict VRSA lacking the van A gene.
American Psychological Association (APA)
Abd al-Hamid, Rashad A.& Ayyub, Nidal S.. 2014. Vancomycin resistance among methicillin resistant staphylococcus aureus isolates from general hospitals. al-Kindy College Medical Journal،Vol. 10, no. 1, pp.80-84.
https://search.emarefa.net/detail/BIM-413697
Modern Language Association (MLA)
Abd al-Hamid, Rashad A.& Ayyub, Nidal S.. Vancomycin resistance among methicillin resistant staphylococcus aureus isolates from general hospitals. al-Kindy College Medical Journal Vol. 10, no. 1 (2014), pp.80-84.
https://search.emarefa.net/detail/BIM-413697
American Medical Association (AMA)
Abd al-Hamid, Rashad A.& Ayyub, Nidal S.. Vancomycin resistance among methicillin resistant staphylococcus aureus isolates from general hospitals. al-Kindy College Medical Journal. 2014. Vol. 10, no. 1, pp.80-84.
https://search.emarefa.net/detail/BIM-413697
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 83-84
Record ID
BIM-413697