Usage of new decamer primer MB1-9 to discriminate six Barbus fish species (Cyprinidae : Teleostei)‎ in Iraqi freshwaters

Abstract EN

The most similar commercial six Barbus fish species in Iraqi waters are Barbus xanthopterus, B.

kersin, B.

barbulus, B.

grypus, B.

sharpey and B.

luteus.

To discriminate molecularly among them using random amplification polymorphic DNA with in polymerase chain reaction (RAPD-PCR) technique.

Single decamer primer MBl-9 is designed locally to scan the genome and amplify DNA fragments.

The PCR products are tested on 2 % agarose gel electrophoresis with 100 bp ladder (standard molecular marker).

The result of the study revealed that the six Barbus spp.

Responded to the primer and gave different patern while the profile of DNA fragments showed 26 scorable bands for all six Barbus spp.

and Cyprinus carpio distributed from one to six bands per species while their sizes ranged from 64 bp which we can consider it as dignostic band to this group till 807 bp in B.

barbulus.

The approximate values of DNA fragments calculated by using the linear equation of trendline of the standard curve plot between natural logarithm of 100 bp ladder bands size versus the distance migrated by the bands.

In addition histogram is drawn as profile analog to the DNA fragment.

The study concluded that this single decamere primer MBl-9 is suitable to discriminate among the most similer six native Barbus spp.

and to confirm RAPD-PCR technique is efficient to differentiate among Iraqi freshwater fish species.