In vitro anthelmintic and cytotoxic activities of extracts from the stem barks of Berlinia confusa (C. Hoyle)‎ and identification of its active constituents

Abstract EN

Phytochemical investigation of EtOAc extract from the stem bark of Berlinia confuse yielded a new and two known polysaturated monoacylglycerides characterised as 1-O-docosanoyl-sn-glyceride (3), 1-O-(13-methyltetradecanoyl)-sn-glycerol (4) and 1-O-pentadecacanoyl-snglycerol (5) ; along with the known compounds betulinic acid (1) and sitosteryl-b-D-glucoside (2).

The structures of these compounds were elucidated using analytical methods, including 1D and 2D-NMR together with MS spectroscopy.

The extracts and isolated compounds demonstrated concentration- dependent anthelmintic activities against Fusciola gigantica (liver flukes) and Taenia solium (tapeworm) at 10–100 mg / ml.

The extracts and isolated compounds were evaluated for cytotoxicity against a small panel of three human tumour cell lines.