Multiplex PCR and latex agglutination for identification and characterization of toxigenic clostridium difficile from raw milk
Other Title(s)
تفاعل البلمرة المتسلسل المتعدد و اختبار التجلط للتعرف على و تمييز الكلوستريديام ديفيسيل في اللبن الخام
Joint Authors
Sad, Najah Muhamad
Amir, Amr Abd al-Mumin
al-Labudi, Ahlam Amin
al-Muaththin, Nuha S.
Source
Assiut Veterinary Medical Journal
Issue
Vol. 60, Issue 143 (31 Oct. 2014), pp.49-53, 5 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2014-10-31
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Topics
Abstract AR
تعتبر الكلوستريديام ديفيسيل من الميكروبات اللاهوائية و التي تؤدى إلى الحالات المعوية و التي من أخطرها ثقب القولون و في هذه الدراسة تم جمع خمسين عينة عشوائية من اللبن الخام المباع في الأسواق، و أوضحت النتائج أن 11 عينة (22%) كانت إيجابية للكلوستريديام ديفيسيل و ذلك باستخدام الطرق التقليدية.
بينما كانت هناك عترتان إيجابيتان للميكروب باستخدام اختبار التجلط، أما باستخدام اختبار البلمرة المتسلسل المتعدد أثبتت النتائج أن (50%) من العترات المختبرة كانت إيجابية و التي لم تكن بدورها مفرزة للسم B.
و بذلك اثبت تفاعل البلمرة المتسلسل المتعدد انه طريقة سريعة و حساسة للكشف عن ميكروب الكلوستريديام ديفيسيل مقارنة بالطرق التقليدية.
هذا و قد تم ذكر الشروط الصحية الواجب إتباعها لمنع تلوث الألبان بهذا الميكروب.
Abstract EN
Community-acquired C.
Diff.
infections have recently been increasing in incidence and severity.
Several studies have isolated C.
Diff.
spores from livestock and food suggesting that food may play a role in transmission.
The overall goals of this research were to : 1) determine prevalence of C.
Diff.
from raw milk using selective (cycloserine-cefoxitin fructose agar [CCFA] ; 2) determine toxigenic profiles of C.
Diff.
isolated using PCR targeting tpi and tcdB genes.
A total of 50 random samples of raw marketable milk were collected from different localities in Assuit city, Egypt.
The obtained results indicated that about 11 (22 %) out of 50 examined raw milk samples were positive for C.
Diff.
using conventional method.
While, 2 strains of C.
Diff.
(18.2 %) out of the positive 11 strains could be detected using the latex agglutination test.
The prevelance rate of C.
Diff using multiplex PCR was one (50 %) out of the two tested strains containing the DNA of C.
Diff., which was negative for toxin B production.
The public health concern of C.
Diff and the suggestive measures were disscused.
The prescence of toxigenic C.
Diff.
in raw milk is of great concern and suggests its transmission through milk, which needs proper milking and handling to reduce risk to the public health.
American Psychological Association (APA)
al-Labudi, Ahlam Amin& Sad, Najah Muhamad& Amir, Amr Abd al-Mumin& al-Muaththin, Nuha S.. 2014. Multiplex PCR and latex agglutination for identification and characterization of toxigenic clostridium difficile from raw milk. Assiut Veterinary Medical Journal،Vol. 60, no. 143, pp.49-53.
https://search.emarefa.net/detail/BIM-427470
Modern Language Association (MLA)
al-Labudi, Ahlam Amin…[et al.]. Multiplex PCR and latex agglutination for identification and characterization of toxigenic clostridium difficile from raw milk. Assiut Veterinary Medical Journal Vol. 60, no. 143 (Oct. 2014), pp.49-53.
https://search.emarefa.net/detail/BIM-427470
American Medical Association (AMA)
al-Labudi, Ahlam Amin& Sad, Najah Muhamad& Amir, Amr Abd al-Mumin& al-Muaththin, Nuha S.. Multiplex PCR and latex agglutination for identification and characterization of toxigenic clostridium difficile from raw milk. Assiut Veterinary Medical Journal. 2014. Vol. 60, no. 143, pp.49-53.
https://search.emarefa.net/detail/BIM-427470
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 52-53
Record ID
BIM-427470