Application of response surface method to evaluate the cytotoxic potency of Ulva fasciata delile, a marine macro alga
Joint Authors
Das, Mukesh Kumar
Sahu, Prafulla Kumar
Rao, G. Srinivasa
Mukkanti, K.
Silpavathi, L.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 21, Issue 6 (31 Dec. 2014), pp.539-546, 8 p.
Publisher
Publication Date
2014-12-31
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Topics
Abstract EN
Bioprospecting of marine natural products has recently produced a substantial number of drug candidates.
Ulva fasciata Delile, belonging to the family Ulvaceae, is a green marine macro alga that grows profusely on the coastal seashore of South India.
In the present study, we investigated the in vitro cytotoxic potential of a methanolic extract of U.
fasciata Delile (MEUF) using a 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay against human colon carcinoma (HT-29), human hepatocyte carcinoma (Hep-G2), and human breast carcinoma (MCF-7) cell lines.
Response surface methodology (RSM) was applied using central-composite experimental design (CCD) to obtain optimum combined effect of concentration and cancer cells with highest cytotoxicity.
The effect of concentration, cancer cell lines as independent variables on absorbance (OD), percent cell survival and percent cell inhibition as dependent variables was investigated.
Maximum cytotoxic activity of MEUF was established for Hep-G2 with lowest OD or percent cell survival ; highest percent cell inhibition with significant difference (p > 0.05) was compared to HT-29 and MCF-7.
American Psychological Association (APA)
Das, Mukesh Kumar& Sahu, Prafulla Kumar& Rao, G. Srinivasa& Mukkanti, K.& Silpavathi, L.. 2014. Application of response surface method to evaluate the cytotoxic potency of Ulva fasciata delile, a marine macro alga. Saudi Journal of Biological Sciences،Vol. 21, no. 6, pp.539-546.
https://search.emarefa.net/detail/BIM-427811
Modern Language Association (MLA)
Das, Mukesh Kumar…[et al.]. Application of response surface method to evaluate the cytotoxic potency of Ulva fasciata delile, a marine macro alga. Saudi Journal of Biological Sciences Vol. 21, no. 6 (Dec. 2014), pp.539-546.
https://search.emarefa.net/detail/BIM-427811
American Medical Association (AMA)
Das, Mukesh Kumar& Sahu, Prafulla Kumar& Rao, G. Srinivasa& Mukkanti, K.& Silpavathi, L.. Application of response surface method to evaluate the cytotoxic potency of Ulva fasciata delile, a marine macro alga. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 6, pp.539-546.
https://search.emarefa.net/detail/BIM-427811
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 546
Record ID
BIM-427811