Genetic variability of cotton leaf curl betasatellite in Northern India
Joint Authors
Sohrab, Sayyid Sartaj
Azhar, Isam I.
Kamal, Muhammad A.
Bhattacharya, P. S.
Rana, D.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 21, Issue 6 (31 Dec. 2014), pp.626-631, 6 p.
Publisher
Publication Date
2014-12-31
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
Cotton is an important crop and its production is affected by various disease pathogens.
Monopartite begomovirus associated betasatellites cause Cotton leaf curl disease (CLCuD) in Northern India.
In order to access the occurrence and genetic variability of Cotton leaf curl betasatellites, an extensive field survey was conducted in states of Rajasthan, Punjab and Haryana.
We selected the betasatellite sequence for analysis as they are reported as important for disease severity and sequence variability.
Based on the field observations, the disease incidence ranged from 30% to 80 % during the survey.
Full genome and DNA b were amplified from various samples while no amplicon was obtained in some samples.
The nucleotide sequence homology ranged from 90.0 % to 98.7 % with Cotton leaf curl virus (CLCuV), 55.2-55.5 % with Bhendi yellow vein mosaic virus, 55.8 % with Okra leaf curl virus and 51.70% with Tomato leaf curl virus isolates.
The lowest similarity (47.8 %) was found in CLCuV-Sudan isolate.
Phylogenetic analysis showed that analyzed isolates formed a close cluster with various CLCuV isolates reported earlier.
The analysis results show sequence variation in Cotton leaf curl betasatellite which could be the result of recombination.
The results obtained by genome amplification and sequence variability indicate that some new variants are circulating and causing leaf curl disease in Rajasthan, Punjab and Haryana.
American Psychological Association (APA)
Sohrab, Sayyid Sartaj& Azhar, Isam I.& Kamal, Muhammad A.& Bhattacharya, P. S.& Rana, D.. 2014. Genetic variability of cotton leaf curl betasatellite in Northern India. Saudi Journal of Biological Sciences،Vol. 21, no. 6, pp.626-631.
https://search.emarefa.net/detail/BIM-427873
Modern Language Association (MLA)
Sohrab, Sayyid Sartaj…[et al.]. Genetic variability of cotton leaf curl betasatellite in Northern India. Saudi Journal of Biological Sciences Vol. 21, no. 6 (Dec. 2014), pp.626-631.
https://search.emarefa.net/detail/BIM-427873
American Medical Association (AMA)
Sohrab, Sayyid Sartaj& Azhar, Isam I.& Kamal, Muhammad A.& Bhattacharya, P. S.& Rana, D.. Genetic variability of cotton leaf curl betasatellite in Northern India. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 6, pp.626-631.
https://search.emarefa.net/detail/BIM-427873
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 630-631
Record ID
BIM-427873