طريقة جديدة لتحديد القدرة الإمراضية لعزلات من فطر Fusarium oxysporum f.sp. lentis

Abstract EN

This study aimed to evaluate three methods of inoculation, (i) inoculation of lentil seedlings grown in test tubes containing Hoagland semi-solid medium, (ii)seedling inoculation with small disks of colony isolates, (iii) seedlings irrigated with spore suspension in pots, iv) coating wheat grains with the pathogen.

The advantages and disadvantages of each method were evaluated in order to select the best one to screen the isolates for pathogenicity.

Results showed significant differences between the methods studied in the early seedling stage, but differences were not significant at the advanced plant stage.

Results of isolates characterization for pathogenicity were close to each other in all methods used, with preference of coating wheat grains with the pathogen.