Flow injection spectrophotometric determination of tetracycline hydrochloride in pharmaceutical samples

Abstract EN

Tetracycline hydrochloride (TCH) was determined spectrophoto-metrically in the pure form and in the pharmaceutical formulations using flow injection analysis (FIA).

The automated method is based on the reaction between TCH, sodium nitroprusside and hydroxylamine hydrochloride in alkaline medium.

The product absorbed maximally at 529 nm.

The calibration graph of TCH is linear from 25-1000 μg mL-1 with detection limit (S / N=3) of 7.589 μg mL-1 and sample through put of 69 h-1.

The various chemical and physical variables were optimized and the proposed procedure was applied successfully for estimation of drug in different commercial forms.