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Differential effects of anti-cancer and anti-hepatitis drugs on liver cystatin
Joint Authors
Atif, Muhammad
Khan, Mohd Shahnawaz
Tabrez, Shams
Shah, Alya
Priyadarshini, Medha
Amin, Fakhra
Zahir, Jalilah F.
Bano, Balqis
Source
Saudi Journal of Biological Sciences
Issue
Vol. 22, Issue 1 (31 Mar. 2015), pp.69-74, 6 p.
Publisher
Publication Date
2015-03-31
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
The drug-protein interaction has been the subject of increasing interest over the decades.
In the present communication, the interaction of liver cystatin with anti-cancer (adriamycin) and anti-hepatitis (adevofir dipivoxil) drugs was studied by thiol-protease inhibitory assay, UV absorption, fluorescence spectroscopy and circular dichroism (CD).
A static type of quenching was observed between the protein and the drug molecules.
Binding constant (Ka) of adriamycin to liver cystatin (LC) was found to be 1.08 × 106 M-1.
Moreover, binding site number was found to be 2.
Importantly, cystatin loses its activity in the presence of adriamycin.
However, intrinsic fluorescence studies in the presence of adevofir dipivoxil showed enhancement in the fluorescence intensity suggesting that binding of adevofir to LC caused unfolding of the protein.
The unfolding of the test protein was also accompanied by significant loss of inhibitory activity.
CD spectroscopy result showed, both adriamycin and adevofir dipivoxil caused perturbation in the secondary structure of liver cystatin.
The possible implications of these results will help in combating drug induced off target effects.
American Psychological Association (APA)
Shah, Alya& Priyadarshini, Medha& Khan, Mohd Shahnawaz& Atif, Muhammad& Amin, Fakhra& Tabrez, Shams…[et al.]. 2015. Differential effects of anti-cancer and anti-hepatitis drugs on liver cystatin. Saudi Journal of Biological Sciences،Vol. 22, no. 1, pp.69-74.
https://search.emarefa.net/detail/BIM-437344
Modern Language Association (MLA)
Shah, Alya…[et al.]. Differential effects of anti-cancer and anti-hepatitis drugs on liver cystatin. Saudi Journal of Biological Sciences Vol. 22, no. 1 (2015), pp.69-74.
https://search.emarefa.net/detail/BIM-437344
American Medical Association (AMA)
Shah, Alya& Priyadarshini, Medha& Khan, Mohd Shahnawaz& Atif, Muhammad& Amin, Fakhra& Tabrez, Shams…[et al.]. Differential effects of anti-cancer and anti-hepatitis drugs on liver cystatin. Saudi Journal of Biological Sciences. 2015. Vol. 22, no. 1, pp.69-74.
https://search.emarefa.net/detail/BIM-437344
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 74
Record ID
BIM-437344