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An Accelerated method for the detection of extended-spectrum B-lactamases in urinary isolates of echerichia and klebsiella penumoniae
Joint Authors
Qadir, Abd Al-Rahman A.
Kumar, Angamuthu
Krishna, Ananth
Zaman, Muhammad Nassimu
Source
Saudi Journal of Kidney Diseases and Transplantation
Issue
Vol. 17, Issue 4 (31 Aug. 2006), pp.535-539, 5 p.
Publisher
Saudi Center for Organ Transplantation
Publication Date
2006-08-31
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
We prospectively studied an accelerated phenotypic method by incorporating the double disk synergy test in the standard Kirby-Bauer disk diffusion susceptibility testing, to evaluate a protocol for the rapid detection of extended-spectrum B-lactamases (ESBL) in urinary isolates of Escherichia coli (E.
coli) and Klebsiella pneumoniae (K.
pneumoniae).
All ESBL-positive isolates were confirmed by the standard Clinical Laboratory Standards Institute (CLSI) confirmatory disk diffusion method.
Between November 2004 and December 2005, a total of 6988 urine specimens were analyzed of which, 776 (11 %) showed significant growth.
They included E.
coli in 577 cases (74 %) and K.
pneumoniae in 199 (25.6 %).
Of these, 63 E.
coli (8 %) and 15 K.
pneumoniae (7.5 %) were positive for ESBL by the accelerated and CLSI methods.
Compared to the standard CLSI method, the accelerated method reduced the ESBL detection time from two days to one day.
We conclude that the accelerated ESBL detection technique used by us in this study is a reliable and rapid method for detecting ESBL in urinary isolates of E.
coli and K.
pneumonia.
American Psychological Association (APA)
Qadir, Abd Al-Rahman A.& Kumar, Angamuthu& Krishna, Ananth& Zaman, Muhammad Nassimu. 2006. An Accelerated method for the detection of extended-spectrum B-lactamases in urinary isolates of echerichia and klebsiella penumoniae. Saudi Journal of Kidney Diseases and Transplantation،Vol. 17, no. 4, pp.535-539.
https://search.emarefa.net/detail/BIM-43780
Modern Language Association (MLA)
Qadir, Abd Al-Rahman A.…[et al.]. An Accelerated method for the detection of extended-spectrum B-lactamases in urinary isolates of echerichia and klebsiella penumoniae. Saudi Journal of Kidney Diseases and Transplantation Vol. 17, no. 4 (Dec. 2006), pp.535-539.
https://search.emarefa.net/detail/BIM-43780
American Medical Association (AMA)
Qadir, Abd Al-Rahman A.& Kumar, Angamuthu& Krishna, Ananth& Zaman, Muhammad Nassimu. An Accelerated method for the detection of extended-spectrum B-lactamases in urinary isolates of echerichia and klebsiella penumoniae. Saudi Journal of Kidney Diseases and Transplantation. 2006. Vol. 17, no. 4, pp.535-539.
https://search.emarefa.net/detail/BIM-43780
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 538-539
Record ID
BIM-43780