Detection of endotoxins in escherichia coli of dairy calves in Khartoum State, Sudan

Abstract EN

A total of 260 E.

coli were isolated from faecal samples collected from 300 diarrhoeic calves in different localities of Khartoum State.

These isolates were subjected for further confirmation by using API 20E and VITEK2 systems.

The ability of some E.

coli to produce enterotoxins was detected by using Suckling Mouse Test (SMT) and Reversed Passive Latex Agglutination (RPLA) test.

Ninety isolates were confirmed by API 20E strips.

Different identification percentages ranging from 89.8 % to 99.8 % were obtained by API 20E strips.

Twenty E.

coli scored high probability percentages ranging from 96 % to 99 % by using VITEK2.

This is the first report in Sudan of using VITEK2 automated identification system for identification of E.

coli.

Fifty E.

coli were selected randomly and examined for their production of heat-stable enterotoxin (STa) by using SMT.

Forty five isolates (90.0 %) were positive.

These 50 E.

coli were subjected to LT enterotoxins production ability test by using RPLA kit.

This is the first report in Sudan of detection of LT in E.

coli isolated from diarrhoeic dairy calves by using RPLA kits.