Regulation Effect of Zinc Fingers and Homeoboxes 2 on Alpha-Fetoprotein in Human Hepatocellular Carcinoma
Joint Authors
Wen, Jian-Ming
Hu, Shao Wei
Xue, Ling
Zhang, Meng
Source
Gastroenterology Research and Practice
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Aim.
To investigate the relationship between alpha-fetoprotein and zinc fingers and homeoboxes 2 in hepatocellular carcinoma.
Materials and Methods.
The expressions of zinc fingers and homeoboxes 2, nuclear factor-YA, and alpha-fetoprotein mRNA in 63 hepatocellular carcinoma were detected by reverse transcriptase-polymerase chain reaction and compared with the clinical parameters of the patients.
Selectively, silence of zinc fingers and homeoboxes 2 in HepG2 cells was detected by RNA interference technique.
Results.
Alpha-fetoprotein mRNA expression was detected in 60.3% of hepatocellular carcinoma cases.
Zinc fingers and homeoboxes 2 mRNA expression (36.5%) was significantly negatively correlated with serum alpha-fetoprotein concentration and mRNA expression.
A strong positive correlation was found between zinc fingers and homeoboxes 2 and nuclear factor-YA mRNA expression (42.9%), while the latter was negatively correlated with serum alpha-fetoprotein concentration and mRNA expression.
Treatment with zinc fingers and homeoboxes 2 small interfering RNA led to 85% and 83% silence of zinc fingers and homeoboxes 2 mRNA and protein expression and 60% and 61% reduction of nuclear factor-YA mRNA and protein levels in the HepG2 cells, respectively.
Downregulation of zinc fingers and homeoboxes 2 also induced a 2.4-fold increase in both alpha-fetoprotein mRNA and protein levels.
Conclusions.
Zinc fingers and homeoboxes 2 can regulate alpha-fetoprotein expression via the interaction with nuclear factor-YA in human hepatocellular carcinoma and may be used as an adjuvant diagnostic marker for alpha-fetoprotein-negative hepatocellular carcinoma.
American Psychological Association (APA)
Hu, Shao Wei& Zhang, Meng& Xue, Ling& Wen, Jian-Ming. 2013. Regulation Effect of Zinc Fingers and Homeoboxes 2 on Alpha-Fetoprotein in Human Hepatocellular Carcinoma. Gastroenterology Research and Practice،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-446354
Modern Language Association (MLA)
Hu, Shao Wei…[et al.]. Regulation Effect of Zinc Fingers and Homeoboxes 2 on Alpha-Fetoprotein in Human Hepatocellular Carcinoma. Gastroenterology Research and Practice No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-446354
American Medical Association (AMA)
Hu, Shao Wei& Zhang, Meng& Xue, Ling& Wen, Jian-Ming. Regulation Effect of Zinc Fingers and Homeoboxes 2 on Alpha-Fetoprotein in Human Hepatocellular Carcinoma. Gastroenterology Research and Practice. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-446354
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446354