Bioanalytical Method Development and Validation of Memantine in Human Plasma by High Performance Liquid Chromatography with Tandem Mass Spectrometry : Application to Bioequivalence Study

Abstract EN

A simple, sensitive, and rapid HPLC-MS/MS method was developed and validated for quantitative estimation of memantine in human plasma.

Chromatography was performed on Zorbax SB-C18 (4.6×75 mm, 3.5 μm) column.

Memantine (ME) and internal standard Memantine-d6(MED6) were extracted by using liquid-liquid extraction and analyzed by LC-ESI-MS/MS using multiple-reaction monitoring (MRM) mode.

The assay exhibited a linear dynamic range of 50.00–50000.00 pg/ml for ME in human plasma.

This method demonstrated an intra- and interday precision within the range of 2.1–3.7 and 1.4–7.8%, respectively.

Further intra- and interday accuracy was within the range of 95.6–99.8 and 95.7–99.1% correspondingly.

The mean recovery of ME and MED6 was 86.07±6.87 and 80.31±5.70%, respectively.

The described method was successfully employed in bioequivalence study of ME in Indian male healthy human volunteers under fasting conditions.