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Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries
Joint Authors
Cremonesi, Fausto
Lange-Consiglio, A.
Anderson, K.
Source
Veterinary Medicine International
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-05
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Two methods have been described to recover oocytes from equine follicles in excised ovaries: aspiration and scraping.
Aim of this work was to develop an effective method for collecting equine oocytes using Tuohy needle and comparing this technique to aspiration and scraping, with or without tunica albuginea removal.
This hollow hypodermic needle, usually employed for inserting epidural catheters, is designed with a slightly curved tip, shaped similar to a small curette.
In unpeeled ovaries, the recovery rates of Tuohy needle group was higher (P<.05) than in the 16 g needle aspiration and in the scraped ovaries (57% versus 36% and 47%) while the rate of cumulus-intact oocytes was higher than aspiration (46.9% versus 39.36%) but lower than scraping (46.97%) (P<.001).
In unpeeled ovaries there was significant difference in maturation rate of oocytes recovered by Tuohy needle in respect to peeled ovaries (58.54% versus 50.17%, resp.).
Combination of aspiration and scraping by Tuohy needle allows a faster and reliable collection of oocytes suitable for horse IVM.
American Psychological Association (APA)
Cremonesi, Fausto& Anderson, K.& Lange-Consiglio, A.. 2010. Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries. Veterinary Medicine International،Vol. 2010, no. 2010, pp.1-4.
https://search.emarefa.net/detail/BIM-446482
Modern Language Association (MLA)
Cremonesi, Fausto…[et al.]. Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries. Veterinary Medicine International No. 2010 (2010), pp.1-4.
https://search.emarefa.net/detail/BIM-446482
American Medical Association (AMA)
Cremonesi, Fausto& Anderson, K.& Lange-Consiglio, A.. Efficacy of Tuohy Needle in Oocytes Collection from Excised Mare Ovaries. Veterinary Medicine International. 2010. Vol. 2010, no. 2010, pp.1-4.
https://search.emarefa.net/detail/BIM-446482
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446482