Highly Sensitive Detection of Organophosphate Insecticides Using Biosensors Based on Genetically Engineered Acetylcholinesterase and Poly(3,4-Ethylenedioxythiophene)
Joint Authors
Ochoteco, Estibalitz
Istamboulie, Georges
Noguer, Thierry
Jubete, Elena
Sikora, Tomasz
Marty, Jean-Louis
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A poly(3,4-ethylenedioxythiophene) (PEDOT) conducting ink is presented as a new electroactive material to be incorporated in acetylcholinesterase-(AChE-) based screen printed biosensors, acting not only as a conducting template but also as an electrochemical mediator for thiocholine oxidation.
Two different strategies have been studied for the chemical synthesis of PEDOT: (a) a classical oxidative polymerisation and (b) a more innovative enzymatic polymerisation, giving a water-soluble PEDOT.
The use of this water-soluble conducting polymer as mediator in screen-printed biosensors enables its deposition by printing like the rest of the layers.
Highly sensitive acetylcholinesterase-(AChE-) based screen-printed biosensors have been constructed using both classical and enzymatic PEDOT, in combination with genetically modified AChE.
These electrodes allow the measurement of thiocholine oxidation at potentials of 100 mV versus Ag/AgCl reference electrode through the mediation of PEDOT.
Inhibition of thiocholine production in presence of CPO allow for detection of this pesticide in concentrations as low as 1⋅10−10 M.
American Psychological Association (APA)
Sikora, Tomasz& Istamboulie, Georges& Jubete, Elena& Ochoteco, Estibalitz& Marty, Jean-Louis& Noguer, Thierry. 2011. Highly Sensitive Detection of Organophosphate Insecticides Using Biosensors Based on Genetically Engineered Acetylcholinesterase and Poly(3,4-Ethylenedioxythiophene). Journal of Sensors،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-446511
Modern Language Association (MLA)
Sikora, Tomasz…[et al.]. Highly Sensitive Detection of Organophosphate Insecticides Using Biosensors Based on Genetically Engineered Acetylcholinesterase and Poly(3,4-Ethylenedioxythiophene). Journal of Sensors No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-446511
American Medical Association (AMA)
Sikora, Tomasz& Istamboulie, Georges& Jubete, Elena& Ochoteco, Estibalitz& Marty, Jean-Louis& Noguer, Thierry. Highly Sensitive Detection of Organophosphate Insecticides Using Biosensors Based on Genetically Engineered Acetylcholinesterase and Poly(3,4-Ethylenedioxythiophene). Journal of Sensors. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-446511
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446511