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Development and Validation of a Reversed-Phase Liquid Chromatography Method for the Simultaneous Determination of Indole-3-Acetic Acid, Indole-3-Pyruvic Acid, and Abscisic Acid in Barley (Hordeum vulgare L.)
Joint Authors
Rostoks, Nils
Keisa, Anete
Nakurte, Ilva
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A simple, sensitive, precise, and specific reverse HPLC method was developed and validated for the determination of plant hormones in barley (Hordeum vulgare L.).
The method includes extraction in aqueous organic solvent followed by solid-phase extraction, sample evaporation, and reversed-phase HPLC analysis in a general purpose UV-visible (abscisic acid (ABA)) and fluorescence detection (indole-3-acetic acid (IAA) and indole-3-pyruvic acid (IPA)), high-performance liquid chromatography system.
The separation was carried out on Zorbax Eclipse XDB C8 column (150 × 4.6 mm I.D) with a mobile phase composed of methanol and 1% acetic acid (60 : 40 v/v) in isocratic mode at a flow rate of 1 ml min-1.
The detection was monitored at 270 nm (ABA) and at 282 nm (Ex) and 360 nm (Em) (IAA, IPA).
The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification, and robustness.
The determined validation parameters are in the commonly acceptable ranges for that kind of analysis.
American Psychological Association (APA)
Nakurte, Ilva& Keisa, Anete& Rostoks, Nils. 2012. Development and Validation of a Reversed-Phase Liquid Chromatography Method for the Simultaneous Determination of Indole-3-Acetic Acid, Indole-3-Pyruvic Acid, and Abscisic Acid in Barley (Hordeum vulgare L.). Journal of Analytical Methods in Chemistry،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-446580
Modern Language Association (MLA)
Nakurte, Ilva…[et al.]. Development and Validation of a Reversed-Phase Liquid Chromatography Method for the Simultaneous Determination of Indole-3-Acetic Acid, Indole-3-Pyruvic Acid, and Abscisic Acid in Barley (Hordeum vulgare L.). Journal of Analytical Methods in Chemistry No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-446580
American Medical Association (AMA)
Nakurte, Ilva& Keisa, Anete& Rostoks, Nils. Development and Validation of a Reversed-Phase Liquid Chromatography Method for the Simultaneous Determination of Indole-3-Acetic Acid, Indole-3-Pyruvic Acid, and Abscisic Acid in Barley (Hordeum vulgare L.). Journal of Analytical Methods in Chemistry. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-446580
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446580