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Upregulation of Salmonella-Induced IL-6 Production in Caco-2 Cells by PJ-34, PARP-1 Inhibitor : Involvement of PI3K, p38 MAPK, ERK, JNK, and NF-κB
Author
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Following Salmonella invasion, intestinal epithelial cells release a distinct array of proinflammatory cytokines.
Interleukin (IL)-6 produced by enterocytes may have anti-inflammatory and cell-protective effects, and may counteract some of the injurious effects of sepsis and endotoxemia.
Recent studies in a variety of rodent models of experimental colitis by using PJ-34, a potent poly (ADP-ribose) polymerase-1 (PARP-1) inhibitor, support the concept that the marked beneficial effect of PJ-34 can be exploited to treat human inflammatory diseases.
The present study was to investigate the effect of PJ-34 on Salmonella-induced enterocyte IL-6 production and its mechanisms.
We found that PJ-34 enhanced Salmonella-induced IL-6 production in Caco-2 cells, either secreted protein or mRNA expression.
PJ-34 treatment enhanced the activity of NF-κB in Salmonella-infected Caco-2 cells.
Besides, the involvement of PJ-34 in up-regulating IL-6 production in S.
typhimurium-infected Caco-2 cells might be also through the ERK but not p38 MAPK, JNK or PI3K/Akt pathways, as demonstrated by Western blot of phosphorylated ERK, p38, JNK and Akt proteins.
It suggests that PJ-34 may exert its protective effect on intestinal epithelial cells against invasive Salmonella infection by up-regulating IL-6 production through ERK and NF-κB but not P38 MAPK, JNK or PI3K/Akt signal pathways.
American Psychological Association (APA)
Huang, Fu-Chen. 2010. Upregulation of Salmonella-Induced IL-6 Production in Caco-2 Cells by PJ-34, PARP-1 Inhibitor : Involvement of PI3K, p38 MAPK, ERK, JNK, and NF-κB. Mediators of Inflammation،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-446624
Modern Language Association (MLA)
Huang, Fu-Chen. Upregulation of Salmonella-Induced IL-6 Production in Caco-2 Cells by PJ-34, PARP-1 Inhibitor : Involvement of PI3K, p38 MAPK, ERK, JNK, and NF-κB. Mediators of Inflammation No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-446624
American Medical Association (AMA)
Huang, Fu-Chen. Upregulation of Salmonella-Induced IL-6 Production in Caco-2 Cells by PJ-34, PARP-1 Inhibitor : Involvement of PI3K, p38 MAPK, ERK, JNK, and NF-κB. Mediators of Inflammation. 2010. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-446624
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446624