Effect of Low-Level Laser on Bone Defects Treated with Bovine or Autogenous Bone Grafts : In Vivo Study in Rat Calvaria

Abstract EN

Objective.

The purpose of this study was to histologically evaluate the effect of low-level laser (LLL) on the healing of critical size defects (CSD) in rat calvaria, filled with autogenous or inorganic bovine bone grafts.

Methods.

Sixty rats were divided into 6 groups (n=10): C (control—filled with blood clot), LLL (low-level laser—GaAlAs, λ 780 nm, 100 mW, 210 J/cm2, Φ 0.05 cm2; 6 J/point), AB (autogenous bone), ABL (autogenous bone + low-level laser), OB (inorganic bovine bone), and OBL (inorganic bovine bone + LLL).

Material and Methods.

The animals were killed after 30 days.

Histological and histometric analyses were performed by light microscopy.

Results.

The groups irradiated with laser, LLL (47.67% ± 8.66%), ABL (39.15% ± 16.72%), and OBL (48.57% ± 28.22%), presented greater area of new bone formation than groups C (9.96% ± 4.50%), AB (30.98% ± 16.59%), and OB (11.36% ± 7.89%), which were not irradiated.

Moreover, they were significantly better than group C (Kruskal-Wallis test followed by Dunn test, P<0.05).

Conclusion.

The laser accelerated the healing of bone defects and the resorption of particles of the graft material.