![](/images/graphics-bg.png)
Seroprevalence Study of Human Brucellosis by Conventional Tests and Indigenous Indirect Enzyme-Linked Immunosorbent Assay
Joint Authors
Krishnamsetty, Prabhudas
Isloor, Srikrishna
Agasthya, Annapurna S.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-01
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Brucellosis is one of the most important reemerging zoonoses in many countries.
Brucellosis is caused by Gram-negative coccobacillus belonging to genus Brucella.
Human brucellosis often makes the diagnosis difficult.
The symptoms and clinical signs most commonly reported are fever, fatigue, malaise, chills, sweats headaches, myalgia, arthralgia, and weight loss.
Some cases have been presented with only joint pain, lower backache, and involuntary limb movement, burning feet, or ischemic heart attacks.
The focus of this work was to develop a highly sensitive and specific indirect ELISA by using smooth lipopolysaccharide antigen of Brucella abortus 99 to detect anti-Brucella antibodies at Project Directorate on Animal Disease Monitoring and Surveillance.
Serum samples collected from 652 individuals in whom fever was not the major symptom but the complaint was of joint pain, headache, lower backache, and so forth, were screened by Rose Bengal plate agglutination test (RBPT) and standard tube agglutination test (STAT).
Subsequent testing of sera by indigenous indirect ELISA detected 20 samples positive (3.6% seroprevalence), and indirect ELISA was found to be more sensitive than RBPT and STAT.
The seroprevalence in South Karnataka was 2.14%, and in North Karnataka it was 0.92%.
American Psychological Association (APA)
Agasthya, Annapurna S.& Isloor, Srikrishna& Krishnamsetty, Prabhudas. 2012. Seroprevalence Study of Human Brucellosis by Conventional Tests and Indigenous Indirect Enzyme-Linked Immunosorbent Assay. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-446661
Modern Language Association (MLA)
Agasthya, Annapurna S.…[et al.]. Seroprevalence Study of Human Brucellosis by Conventional Tests and Indigenous Indirect Enzyme-Linked Immunosorbent Assay. The Scientific World Journal No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-446661
American Medical Association (AMA)
Agasthya, Annapurna S.& Isloor, Srikrishna& Krishnamsetty, Prabhudas. Seroprevalence Study of Human Brucellosis by Conventional Tests and Indigenous Indirect Enzyme-Linked Immunosorbent Assay. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-446661
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446661