Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR
Joint Authors
Joveen-Neoh, Wan Fen
Wong, Clemente Michael Vui Ling
Chong, Ka Lung
Lau, Tiek Ying
Source
Journal of Parasitology Research
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Introduction.
Malaria is currently one of the most prevalent parasite-transmitted diseases caused by parasites of the genus Plasmodium.
Misidentification of human malaria parasites especially P.
knowlesi based on microscopic examination is very common.
The objectives of this paper were to accurately identify the incidence of human malaria parasites in the interior division of Sabah, Malaysian Borneo, based on small subunit ribosomal RNA (ssrRNA) and to determine the misidentification rate in human malaria parasites.
Methods.
Nested PCR was used to detect the presence of human malaria parasites.
A total of 243 blood spot samples from patients who had requested for blood film for malaria parasite (BFMP) analyses were used in this study.
Results.
Nested PCR findings showed that there was no P.
malariae infection while the highest prevalent malaria parasite was P.
knowlesi, followed by P.
vivax, P.
falciparum, and mixed infection.
Only 69.5% of the 243 samples giving consistent nested PCR and microscopic results.
Conclusion.
The preliminary findings from molecular detection of malaria showed that P.
knowlesi was the most prevalent Plasmodium species in the interior division of Sabah.
The findings from this paper may provide a clearer picture on the actual transmission of different Plasmodium species in this region.
American Psychological Association (APA)
Joveen-Neoh, Wan Fen& Chong, Ka Lung& Wong, Clemente Michael Vui Ling& Lau, Tiek Ying. 2011. Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR. Journal of Parasitology Research،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-446667
Modern Language Association (MLA)
Joveen-Neoh, Wan Fen…[et al.]. Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR. Journal of Parasitology Research No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-446667
American Medical Association (AMA)
Joveen-Neoh, Wan Fen& Chong, Ka Lung& Wong, Clemente Michael Vui Ling& Lau, Tiek Ying. Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR. Journal of Parasitology Research. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-446667
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446667