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High Mass Accuracy Phosphopeptide Identification Using Tandem Mass Spectra
Author
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-15
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Phosphoproteomics is a powerful analytical platform for identification and quantification of phosphorylated peptides and assignment of phosphorylation sites.
Bioinformatics tools to identify phosphorylated peptides from their tandem mass spectra and protein sequence databases are important part of phosphoproteomics.
In this work, we discuss general informatics aspects of mass-spectrometry-based phosphoproteomics.
Some of the specifics of phosphopeptide identifications stem from the labile nature of phosphor groups and expanded peptide search space.
Allowing for modifications of Ser, Thr, and Tyr residues exponentially increases effective database size.
High mass resolution and accuracy measurements of precursor mass-to-charge ratios help to restrict the search space of candidate peptide sequences.
The higher-order fragmentations of neutral loss ions enhance the fragment ion mass spectra of phosphorylated peptides.
We show an example of a phosphopeptide identification where accounting for fragmentation from neutral loss species improves the identification scores in a database search algorithm by 50%.
American Psychological Association (APA)
Sadygov, Rovshan G.. 2012. High Mass Accuracy Phosphopeptide Identification Using Tandem Mass Spectra. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-446702
Modern Language Association (MLA)
Sadygov, Rovshan G.. High Mass Accuracy Phosphopeptide Identification Using Tandem Mass Spectra. International Journal of Proteomics No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-446702
American Medical Association (AMA)
Sadygov, Rovshan G.. High Mass Accuracy Phosphopeptide Identification Using Tandem Mass Spectra. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-446702
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446702