Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide
Joint Authors
Nikolic-Kokic, Aleksandra
Blagojevic, Dusko
Spasic, Mihajlo
Milovanovic, Slobodan
Orescanin-Dusic, Zorana
Appiah, Isabella
Radojicic, Ratko
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The uteri, spontaneously active or Ca2+ (6 mM) induced, were allowed to equilibrate, and to inhibit voltage-gated potassium (KV) channels 1 mM 4-amino pyridine (4-AP) was applied for 15 min before adding H2O2 .
H2O2 was added cumulatively: 2 μM, 20 μM, 200 μM, 400 μM, and 3 mM.
Average time for H2O2 concentrations (2, 20, 200, and 400) μM to reach its full effect was 15 min.
H2O2 3 mM had a prolonged effect and therefore was left to act for 30 min.
Two-way ANOVA showed significant differences in time dependency between spontaneous and Ca2+-induced rat uteri after applying 3 mM H2O2 (type of contraction, P=0.0280), but not 400 μM H2O2 (P=0.9271).
Our results indicate that H2O2 oxidises channel intracellular thiol groups and activates the channel, inducing relaxation.
Cell antioxidative defence system quickly activates glutathione peroxidase (GSHPx) defence mechanism but not catalase (CAT) defence mechanism.
Intracellular redox mechanisms repair the oxidised sites and again establish deactivation of KV channels, recuperating contractility.
In conclusion, our results demonstrate that KV channels can be altered in a time-dependent manner by reversible redox-dependent intracellular alterations.
American Psychological Association (APA)
Appiah, Isabella& Nikolic-Kokic, Aleksandra& Orescanin-Dusic, Zorana& Radojicic, Ratko& Milovanovic, Slobodan& Spasic, Mihajlo…[et al.]. 2012. Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-446802
Modern Language Association (MLA)
Appiah, Isabella…[et al.]. Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-446802
American Medical Association (AMA)
Appiah, Isabella& Nikolic-Kokic, Aleksandra& Orescanin-Dusic, Zorana& Radojicic, Ratko& Milovanovic, Slobodan& Spasic, Mihajlo…[et al.]. Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-446802
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446802