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Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-Imaging
Joint Authors
Steyer, Grant J.
Hakimi, Parvin
Wilson, David L.
Gargesha, Madhusudhana
Roy, Debashish
Hanson, Richard W.
Source
International Journal of Biomedical Imaging
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We have developed, for the Case 3D Cryo-imaging system, a specialized, multiscale visualization scheme which provides color-rich volume rendering and multiplanar reformatting enabling one to visualize an entire mouse and zoom in to organ, tissue, and microscopic scales.
With this system, we have anatomically characterized, in 3D, from whole animal to tissue level, a transgenic mouse and compared it with its control.
The transgenic mouse overexpresses the cytosolic form of phosphoenolpyruvate carboxykinase (PEPCK-C) in its skeletal muscle and is capable of greatly enhanced physical endurance and has a longer life-span and reproductive life as compared to control animals.
We semiautomatically analyzed selected organs such as kidney, heart, adrenal gland, spleen, and ovaries and found comparatively enlarged heart, much less visceral, subcutaneous, and pericardial adipose tissue, and higher tibia-to-femur ratio in the transgenic animal.
Microscopically, individual skeletal muscle fibers, fine mesenteric blood vessels, and intestinal villi, among others, were clearly seen.
American Psychological Association (APA)
Roy, Debashish& Gargesha, Madhusudhana& Steyer, Grant J.& Hakimi, Parvin& Hanson, Richard W.& Wilson, David L.. 2010. Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-Imaging. International Journal of Biomedical Imaging،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-446820
Modern Language Association (MLA)
Roy, Debashish…[et al.]. Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-Imaging. International Journal of Biomedical Imaging No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-446820
American Medical Association (AMA)
Roy, Debashish& Gargesha, Madhusudhana& Steyer, Grant J.& Hakimi, Parvin& Hanson, Richard W.& Wilson, David L.. Multi-Scale Characterization of the PEPCK-Cmus Mouse through 3D Cryo-Imaging. International Journal of Biomedical Imaging. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-446820
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446820