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Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal
Joint Authors
Tripathi, Manisha
Liu, Shanshan
Weidow, Brandy
Yamashita, Hironobu
Jourquin, Jerome
Kam, Yoonseok
Quaranta, Vito
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Lysophosphatidic acid (LPA) is a bioactive phospholipid that affects various biological functions, such as cell proliferation, migration, survival, wound healing, and tumor invasion through LPA receptors.
Previously, we reported that LPA induces A431 colony dispersal, accompanied by disruption of cell-cell contacts and cell migration.
However, it remains unclear how LPA affects cell migration and gene expression during A431 colony dispersal.
In this paper, we performed cDNA microarray analysis to investigate this question by comparing gene expression between untreated and LPA-treated A431 cells.
Interestingly, these results revealed that LPA treatment upregulates several TGF-β1 target genes, including laminin-332 (Ln-332) components (α3, β3, and γ2 chains).
Western blot analysis also showed that LPA increased phosphorylation of Smad2, an event that is carried out by TGF-β1 interactions.
Among the genes upregulated, we further addressed the role of Ln-332.
Real-time PCR analysis confirmed the transcriptional upregulation of all α3, β3, and γ2 chains of Ln-332 by LPA, corresponding to the protein level increases revealed by western blot.
Further, the addition of anti-Ln-332 antibody prevented LPA-treated A431 colonies from dispersing.
Taken together, our results suggest that LPA-induced Ln-332 plays a significant role in migration of individual cells from A431 colonies.
American Psychological Association (APA)
Yamashita, Hironobu& Tripathi, Manisha& Jourquin, Jerome& Kam, Yoonseok& Liu, Shanshan& Weidow, Brandy…[et al.]. 2010. Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal. Journal of Oncology،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-446925
Modern Language Association (MLA)
Yamashita, Hironobu…[et al.]. Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal. Journal of Oncology No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-446925
American Medical Association (AMA)
Yamashita, Hironobu& Tripathi, Manisha& Jourquin, Jerome& Kam, Yoonseok& Liu, Shanshan& Weidow, Brandy…[et al.]. Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal. Journal of Oncology. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-446925
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446925