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Expression of a Novel Chimeric Truncated t-PA in CHO Cells Based on in Silico Experiments
Joint Authors
Davoudi, Noushin
Adeli, Ahmad
Hemayatkar, Mehdi
Mahboudi, Fereidoun
Barkhrdari, Farzaneh
Davami, Fatemeh
Azami, Mehrnaz
Majidzadeh-A, Keivan
Sardari, Soroush
Omidi, Maryam
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Tissue plasminogen activator (t-PA) is one of the fibrin-specific serine proteases that play a crucial role in the fibrinolytic system.
The rapid clearance of the drug from the circulation, caused by its active uptake in the liver, has lead to complicated clinical applications.
Different forms of plasminogen activators have been developed to treat thrombotic disease.
Deletion of the first three domains of t-PA by gene manipulation techniques has shown a significant increase in its plasma half life.
In order to compensate the disadvantage of higher bleeding risk, a novel chimeric truncated form of t-PA with 394 amino acids and more fibrin affinity compared to the truncated form was designed to be expressed in Chinese Hamster Ovarian (CHO) cells.
The recombinant chimeric plasminogen activator consists of kringle 2 and serine protease (K2S) domains of t-PA, namely GHRP-SYQ-K2S.
The level of expression was found to be 752 IU/ml with 566,917 IU/mg specific activity, based on amidolytic activity.
The fibrin binding of this novel chimeric truncated t-PA was 86% of the full length t-PA at a fibrinogen concentration of 0.2 mg/ml.
This could be a promising approach with more desirable pharmacodynamic properties compared to existing commercial forms.
American Psychological Association (APA)
Davami, Fatemeh& Sardari, Soroush& Majidzadeh-A, Keivan& Hemayatkar, Mehdi& Barkhrdari, Farzaneh& Omidi, Maryam…[et al.]. 2010. Expression of a Novel Chimeric Truncated t-PA in CHO Cells Based on in Silico Experiments. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-447044
Modern Language Association (MLA)
Davami, Fatemeh…[et al.]. Expression of a Novel Chimeric Truncated t-PA in CHO Cells Based on in Silico Experiments. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-447044
American Medical Association (AMA)
Davami, Fatemeh& Sardari, Soroush& Majidzadeh-A, Keivan& Hemayatkar, Mehdi& Barkhrdari, Farzaneh& Omidi, Maryam…[et al.]. Expression of a Novel Chimeric Truncated t-PA in CHO Cells Based on in Silico Experiments. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-447044
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447044