![](/images/graphics-bg.png)
Cornel Iridoid Glycoside Attenuates Tau Hyperphosphorylation by Inhibition of PP2A Demethylation
Joint Authors
Yu, Jian-chun
Li, Lin
Li, Ya-li
Kuai, Xue-xian
Zhang, Li
Zhang, Lan
Yang, Cui-cui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Aim.
The aim of the present study was to investigate the effect of cornel iridoid glycoside (CIG) on tau hyperphosphorylation induced by wortmannin (WT) and GF-109203X (GFX) and the underlying mechanisms.
Methods.
Human neuroblastoma SK-N-SH cells were preincubated with CIG (50, 100, and 200 µg/ml, resp.) for 24 h and then exposed to 10 µM WT and 10 µM GFX for 3 h after washing out CIG.
Immunohistochemistry was used to observe the microtubular cytoskeleton of the cultured cells.
Western blotting was used to measure the phosphorylation level of tau protein, glycogen synthase kinase 3β (GSK-3β), and protein phosphatase 2A (PP2A).
The activity of PP2A was detected by a biochemical assay.
Results.
Preincubation of CIG significantly attenuated the WT/GFX-induced tau hyperphosphorylation at the sites of Thr205, Thr212, Ser214, Thr217, Ser396, and PHF-1 and improved the damage of morphology and microtubular cytoskeleton of the cells.
CIG did not prevent the decrease in p-AKT-ser473 and p-GSK-3β-ser9 induced by WT/GFX.
However, CIG significantly elevated the activity of PP2A by reducing the demethylation of PP2A catalytic subunit (PP2Ac) at Leu309 and the ratio of PME-1/LCMT in the WT/GFX-treated cells.
The results suggest that CIG may be beneficial to the treatment of AD.
American Psychological Association (APA)
Yang, Cui-cui& Kuai, Xue-xian& Li, Ya-li& Zhang, Li& Yu, Jian-chun& Li, Lin…[et al.]. 2013. Cornel Iridoid Glycoside Attenuates Tau Hyperphosphorylation by Inhibition of PP2A Demethylation. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-447070
Modern Language Association (MLA)
Yang, Cui-cui…[et al.]. Cornel Iridoid Glycoside Attenuates Tau Hyperphosphorylation by Inhibition of PP2A Demethylation. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-447070
American Medical Association (AMA)
Yang, Cui-cui& Kuai, Xue-xian& Li, Ya-li& Zhang, Li& Yu, Jian-chun& Li, Lin…[et al.]. Cornel Iridoid Glycoside Attenuates Tau Hyperphosphorylation by Inhibition of PP2A Demethylation. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-447070
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447070