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Protective Effect of Liposome-Encapsulated Glutathione in a Human Epidermal Model Exposed to a Mustard Gas Analog
Joint Authors
Brannon, Marianne
Paromov, Victor
Kanaparthy, Naga S.
Stone, William L.
Kumari, Sudha
Smith, Milton G.
Yang, Hongsong
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Pharmacy, Health & Medical Sciences
Medicine
Abstract EN
Sulfur mustard or mustard gas (HD) and its monofunctional analog, 2-chloroethyl ethyl sulfide (CEES), or “half-mustard gas,” are alkylating agents that induce DNA damage, oxidative stress, and inflammation.
HD/CEES are rapidly absorbed in the skin causing extensive injury.
We hypothesize that antioxidant liposomes that deliver both water-soluble and lipid-soluble antioxidants protect skin cells from immediate CEES-induced damage via attenuating oxidative stress.
Liposomes containing water-soluble antioxidants and/or lipid-soluble antioxidants were evaluated using in vitro model systems.
Initially, we found that liposomes containing encapsulated glutathione (GSH-liposomes) increased cell viability and attenuated production of reactive oxygen species (ROS) in HaCaT cells exposed to CEES.
Next, GSH-liposomes were tested in a human epidermal model, EpiDerm.
In the EpiDerm, GSH-liposomes administered simultaneously or 1 hour after CEES exposure (2.5 mM) increased cell viability, inhibited CEES-induced loss of ATP and attenuated changes in cellular morphology, but did not reduce caspase-3 activity.
These findings paralleled the previously described in vivo protective effect of antioxidant liposomes in the rat lung and established the effectiveness of GSH-liposomes in a human epidermal model.
This study provides a rationale for use of antioxidant liposomes against HD toxicity in the skin considering further verification in animal models exposed to HD.
American Psychological Association (APA)
Paromov, Victor& Kumari, Sudha& Brannon, Marianne& Kanaparthy, Naga S.& Yang, Hongsong& Smith, Milton G.…[et al.]. 2011. Protective Effect of Liposome-Encapsulated Glutathione in a Human Epidermal Model Exposed to a Mustard Gas Analog. Journal of Toxicology،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-447165
Modern Language Association (MLA)
Paromov, Victor…[et al.]. Protective Effect of Liposome-Encapsulated Glutathione in a Human Epidermal Model Exposed to a Mustard Gas Analog. Journal of Toxicology No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-447165
American Medical Association (AMA)
Paromov, Victor& Kumari, Sudha& Brannon, Marianne& Kanaparthy, Naga S.& Yang, Hongsong& Smith, Milton G.…[et al.]. Protective Effect of Liposome-Encapsulated Glutathione in a Human Epidermal Model Exposed to a Mustard Gas Analog. Journal of Toxicology. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-447165
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447165