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LEPREL1 Expression in Human Hepatocellular Carcinoma and Its Suppressor Role on Cell Proliferation
Joint Authors
Wei, Xuyong
Wang, Jianguo
Zhou, Lin
Liu, Zhikun
Lu, Di
Xu, Xiao
Zhuang, Runzhou
Zheng, Shu-Sen
Xie, Haiyang
Source
Gastroenterology Research and Practice
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Hepatocellular carcinoma (HCC) is one of the most aggressive malignancies worldwide.
It is characterized by its high invasive and metastatic potential.
Leprecan-like 1 (LEPREL1) has been demonstrated to be downregulated in the HCC tissues in previous proteomics studies.
The present study is aimed at a new understanding of LEPREL1 function in HCC.
Methods.
Quantitative RT-PCR, immunohistochemical analysis, and western blot analysis were used to evaluate the expression of LEPREL1 between the paired HCC tumor and nontumorous tissues.
The biology function of LEPREL1 was investigated by Cell Counting Kit-8 (CCK8) assay and colony formation assay in HepG2 and Bel-7402 cells.
Results.
The levels of LEPREL1 mRNA and protein were significantly lower in the HCC tissues as compared to those of the nontumorous tissues.
Reduced LEPREL1 expression was not associated with conventional clinical parameters of HCC.
Overexpression of LEPREL1 in HepG2 and Bel-7402 cells inhibited cell proliferation (P<0.01) and colony formation (P<0.05).
LEPREL1 suppressed tumor cell proliferation through regulation of the cell cycle by downregulation of cyclins.
Conclusions.
Clinical parameters analysis suggested that LEPREL1 was an independent factor in the development of HCC.
The biology function experiments showed that LEPREL1 might serve as a potential tumor suppressor gene by inhibiting the HCC cell proliferation.
American Psychological Association (APA)
Wang, Jianguo& Xu, Xiao& Liu, Zhikun& Wei, Xuyong& Zhuang, Runzhou& Lu, Di…[et al.]. 2013. LEPREL1 Expression in Human Hepatocellular Carcinoma and Its Suppressor Role on Cell Proliferation. Gastroenterology Research and Practice،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-447191
Modern Language Association (MLA)
Wang, Jianguo…[et al.]. LEPREL1 Expression in Human Hepatocellular Carcinoma and Its Suppressor Role on Cell Proliferation. Gastroenterology Research and Practice No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-447191
American Medical Association (AMA)
Wang, Jianguo& Xu, Xiao& Liu, Zhikun& Wei, Xuyong& Zhuang, Runzhou& Lu, Di…[et al.]. LEPREL1 Expression in Human Hepatocellular Carcinoma and Its Suppressor Role on Cell Proliferation. Gastroenterology Research and Practice. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-447191
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447191