Co-Cultures of Pseudomonas aeruginosa and Roseobacter denitrificans Reveal Shifts in Gene Expression Levels Compared to Solo Cultures
Joint Authors
Conway, Crystal A.
Lopez, Jose V.
Esiobu, Nwadiuto
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-01
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Consistent biosynthesis of desired secondary metabolites (SMs) from pure microbial cultures is often unreliable.
In a proof-of-principle study to induce SM gene expression and production, we describe mixed “co-culturing” conditions and monitoring of messages via quantitative real-time PCR (qPCR).
Gene expression of model bacterial strains (Pseudomonas aeruginosa PAO1 and Roseobacter denitrificans Och114) was analyzed in pure solo and mixed cocultures to infer the effects of interspecies interactions on gene expression in vitro, Two P.
aeruginosa genes (PhzH coding for portions of the phenazine antibiotic pathway leading to pyocyanin (PCN) and the RhdA gene for thiosulfate: cyanide sulfurtransferase (Rhodanese)) and two R.
denitrificans genes (BetaLact for metallo-beta-lactamase and the DMSP gene for dimethylpropiothetin dethiomethylase) were assessed for differential expression.
Results showed that R.
denitrificans DMSP and BetaLact gene expression became elevated in a mixed culture.
In contrast, P.
aeruginosa co-cultures with R.
denitrificans or a third species did not increase target gene expression above control levels.
This paper provides insight for better control of target SM gene expression in vitro and bypass complex genetic engineering manipulations.
American Psychological Association (APA)
Conway, Crystal A.& Esiobu, Nwadiuto& Lopez, Jose V.. 2012. Co-Cultures of Pseudomonas aeruginosa and Roseobacter denitrificans Reveal Shifts in Gene Expression Levels Compared to Solo Cultures. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-447208
Modern Language Association (MLA)
Conway, Crystal A.…[et al.]. Co-Cultures of Pseudomonas aeruginosa and Roseobacter denitrificans Reveal Shifts in Gene Expression Levels Compared to Solo Cultures. The Scientific World Journal No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-447208
American Medical Association (AMA)
Conway, Crystal A.& Esiobu, Nwadiuto& Lopez, Jose V.. Co-Cultures of Pseudomonas aeruginosa and Roseobacter denitrificans Reveal Shifts in Gene Expression Levels Compared to Solo Cultures. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-447208
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447208