Prolonged Laccase Production by a Cold and pH Tolerant Strain of Penicillium pinophilum (MCC 1049) Isolated from a Low Temperature Environment
Joint Authors
Pandey, Anita
Jain, Rahul
Dhakar, Kusum
Tamta, Sushma
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-09
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Production of laccase by a cold and pH tolerant strain of Penicillium pinophilum has been investigated under different cultural conditions for up to 35 days of incubation.
The fungus was originally isolated from a low temperature environment under mountain ecosystem of Indian Himalaya.
The estimations were conducted at 3 temperatures (15, 25, and 35°C), a range of pH (3.5–11.5), and in presence of supplements including carbon and nitrogen sources, vitamins, and antibiotics.
Optimum production of laccase was recorded at 25°C (optimum temperature for fungal growth) and 7.5 pH.
The production of enzyme was recorded maximum on day 28 (11.6±0.52 U/L) following a slow decline at day 35 of incubation (10.6±0.80 U/L).
Fructose and potassium nitrate (0.2%) among nutritional supplements, chloramphenicol (0.1%) among antibiotics, and folic acid (0.1%) among vitamins were found to be the best enhancers for production of laccase.
Relatively lower but consistent production of laccase for a longer period is likely to be an ecologically important phenomenon under low temperature environment.
Further, enhancement in production of enzyme using various supplements will be useful for its use in specific biotechnological applications.
American Psychological Association (APA)
Dhakar, Kusum& Jain, Rahul& Tamta, Sushma& Pandey, Anita. 2014. Prolonged Laccase Production by a Cold and pH Tolerant Strain of Penicillium pinophilum (MCC 1049) Isolated from a Low Temperature Environment. Enzyme Research،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-447269
Modern Language Association (MLA)
Dhakar, Kusum…[et al.]. Prolonged Laccase Production by a Cold and pH Tolerant Strain of Penicillium pinophilum (MCC 1049) Isolated from a Low Temperature Environment. Enzyme Research No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-447269
American Medical Association (AMA)
Dhakar, Kusum& Jain, Rahul& Tamta, Sushma& Pandey, Anita. Prolonged Laccase Production by a Cold and pH Tolerant Strain of Penicillium pinophilum (MCC 1049) Isolated from a Low Temperature Environment. Enzyme Research. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-447269
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447269