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Purification and Characterization of Melanogenic Enzyme Tyrosinase from Button Mushroom
Joint Authors
Zaidi, Kamal Uddin
Ali, Ayesha S.
Ali, Sharique A.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Melanogenesis is a biosynthetic pathway for the formation of the pigment melanin in human skin.
A key enzyme, tyrosinase, catalyzes the first and only rate-limiting steps in melanogenesis.
Since the discovery of its melanogenic properties, tyrosinase has been in prime focus and microbial sources of the enzyme are sought.
Agaricus bisporus widely known as the common edible mushroom, it’s taking place in high amounts of proteins, enzyme, carbohydrates, fibers, and low fat contents are frequently cited in the literature in relation to their nutritional value.
In the present study tyrosinase from Agaricus bisporus was purified by ammonium sulphate precipitation, dialysis followed by gel filtration chromatography on Sephadex G-100, and ion exchange chromatography on DEAE-Cellulose; the enzyme was purified, 16.36-fold to give 26.6% yield on total activity in the crude extract and final specific activity of 52.19 U/mg.
The SDS-PAGE electrophoresis showed a migrating protein band molecular weight of 95 kDa.
The purified tyrosinase was optimized and the results revealed that the optimum values are pH 7.0 and temperature 35°C.
The highest activity was reported towards its natural substrate, L-DOPA, with an apparent Km value of 0.933 mM.
This indicated that tyrosinase purified from Agaricus bisporus is a potential source for medical applications.
American Psychological Association (APA)
Zaidi, Kamal Uddin& Ali, Ayesha S.& Ali, Sharique A.. 2014. Purification and Characterization of Melanogenic Enzyme Tyrosinase from Button Mushroom. Enzyme Research،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-447273
Modern Language Association (MLA)
Zaidi, Kamal Uddin…[et al.]. Purification and Characterization of Melanogenic Enzyme Tyrosinase from Button Mushroom. Enzyme Research No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-447273
American Medical Association (AMA)
Zaidi, Kamal Uddin& Ali, Ayesha S.& Ali, Sharique A.. Purification and Characterization of Melanogenic Enzyme Tyrosinase from Button Mushroom. Enzyme Research. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-447273
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447273