Towards Multitarget Testing in Molecular Microbiology
Joint Authors
Steensels, Deborah
Vankeerberghen, Anne
De Beenhouwer, Hans
Source
International Journal of Microbiology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-25
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Advantages of PCR assays over more conventional culture-based diagnostics include significantly higher sensitivities and shorter turnaround times.
They are particularly useful when patient treatment has already been initiated or for specimens that may contain microorganisms that are slow-growing, difficult to culture, or for which culture methods do not exist.
However, due to genome variability, single target testing might lead to false-negative results.
This paper focuses on examples from our own experiences and the literature to provide insight into the limitations of single target testing in molecular biology.
Lessons learned from these experiences include the careful design of diagnostic assays, preferably multitargeted, the importance of investigating the incidence and epidemiology of infection in detail, the frequent participation in appropriate quality assurance schemes, and the importance of continuous attentiveness by investigators when confronted with inconsistent results.
In conclusion, multitargeted testing in microbiological molecular assays should be a rule.
American Psychological Association (APA)
Steensels, Deborah& Vankeerberghen, Anne& De Beenhouwer, Hans. 2013. Towards Multitarget Testing in Molecular Microbiology. International Journal of Microbiology،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-447295
Modern Language Association (MLA)
Steensels, Deborah…[et al.]. Towards Multitarget Testing in Molecular Microbiology. International Journal of Microbiology No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-447295
American Medical Association (AMA)
Steensels, Deborah& Vankeerberghen, Anne& De Beenhouwer, Hans. Towards Multitarget Testing in Molecular Microbiology. International Journal of Microbiology. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-447295
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447295