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Alpinia pricei Rhizome Extracts Induce Cell Cycle Arrest in Human Squamous Carcinoma KB Cells and Suppress Tumor Growth in Nude Mice
Joint Authors
Hseu, You Cheng
Chen, Chih-Sheng
Wang, Sheng-Yang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-18
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Alpinia pricei has been shown to induce apoptosis in human squamous carcinoma (KB) cells.
In this study, we report the effectiveness of the ethanol (70%) extracts of A.
pricei rhizome (AP extracts) in terms of tumor regression as determined using both in vitro cell culture and in vivo athymic nude mice models of KB cells.
We found that the AP extract (25–200 μg/mL) treatment decreased the proliferation of KB cells by arresting progression through the G2/M phase of the cell cycle.
This cell cycle blockade was associated with reductions in cyclin A and B1, Cdc2, and Cdc25C, and increased p21/WAF1, Wee1, p53 and phospho-p53 (p-p53) in a dose- and time-dependent manner.
Moreover, we found that AP extract treatment decreased metalloproteinase-9 (MMP-9) and urokinase plasminogen activator (u-PA) expression, while expression of their endogenous inhibitors, tissue inhibitor of MMP-1 (TIMP-1) and plasminogen activator inhibitor-1 (PAI-1), were increased in KB cells.
Furthermore, AP extract treatment effectively delayed tumor incidence in nude mice inoculated with KB cells and reduced the tumor burden.
AP extract treatment also induced apoptotic DNA fragmentation, as detected by in situ TUNEL staining.
Thus, A.
pricei may possess antitumor activity in human squamous carcinoma (KB) cells.
American Psychological Association (APA)
Hseu, You Cheng& Chen, Chih-Sheng& Wang, Sheng-Yang. 2011. Alpinia pricei Rhizome Extracts Induce Cell Cycle Arrest in Human Squamous Carcinoma KB Cells and Suppress Tumor Growth in Nude Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-447452
Modern Language Association (MLA)
Hseu, You Cheng…[et al.]. Alpinia pricei Rhizome Extracts Induce Cell Cycle Arrest in Human Squamous Carcinoma KB Cells and Suppress Tumor Growth in Nude Mice. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-447452
American Medical Association (AMA)
Hseu, You Cheng& Chen, Chih-Sheng& Wang, Sheng-Yang. Alpinia pricei Rhizome Extracts Induce Cell Cycle Arrest in Human Squamous Carcinoma KB Cells and Suppress Tumor Growth in Nude Mice. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-447452
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447452