Diverse Effects of Glutathione and UPF Peptides on Antioxidant Defense System in Human Erythroleukemia Cells K562
Joint Authors
Kilk, Kalle
Ehrlich, Kersti
Soomets, Ursel
Zilmer, Mihkel
Mahlapuu, Riina
Kairane, Ceslava
Source
International Journal of Peptides
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-15
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The main goal of the present paper was to examine the influence of the replacement of γ-Glu moiety to α-Glu in glutathione and in its antioxidative tetrapeptidic analogue UPF1 (Tyr(Me)-γ-Glu-Cys-Gly), resulting in α-GSH and UPF17 (Tyr(Me)-Glu-Cys-Gly), on the antioxidative defense system in K562 cells.
UPF1 and GSH increased while UPF17 and α-GSH decreased the activity of CuZnSOD in K562 cells, at peptide concentration of 10 μM by 42% and 38% or 35% and 24%, respectively.
After three-hour incubation, UPF1 increased and UPF17 decreased the intracellular level of total GSH.
Additionally, it was shown that UPF1 is not degraded by γ-glutamyltranspeptidase, which performs glutathione breakdown.
These results indicate that effective antioxidative character of peptides does not depend only on the reactivity of the thiol group, but also of the other functional groups, and on the spatial structure of peptides.
American Psychological Association (APA)
Kairane, Ceslava& Mahlapuu, Riina& Ehrlich, Kersti& Kilk, Kalle& Zilmer, Mihkel& Soomets, Ursel. 2012. Diverse Effects of Glutathione and UPF Peptides on Antioxidant Defense System in Human Erythroleukemia Cells K562. International Journal of Peptides،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-447478
Modern Language Association (MLA)
Kairane, Ceslava…[et al.]. Diverse Effects of Glutathione and UPF Peptides on Antioxidant Defense System in Human Erythroleukemia Cells K562. International Journal of Peptides No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-447478
American Medical Association (AMA)
Kairane, Ceslava& Mahlapuu, Riina& Ehrlich, Kersti& Kilk, Kalle& Zilmer, Mihkel& Soomets, Ursel. Diverse Effects of Glutathione and UPF Peptides on Antioxidant Defense System in Human Erythroleukemia Cells K562. International Journal of Peptides. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-447478
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447478