Reprogramming of Human Huntington Fibroblasts Using mRNA
Joint Authors
Nikkhah, Guido
Naaldijk, Yahaira M.
Binder, Hans
Fabian, Claire
Arnold, Antje
Armstrong, Lyle
Stolzing, Alexandra
Wirth, Henry
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-07
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The derivation of induced pluripotent stem cells (iPS) from human cell sources using transduction based on viral vectors has been reported by several laboratories.
Viral vector-induced integration is a potential cause of genetic modification.
We have derived iPS cells from human foreskin, adult Huntington fibroblasts, and adult skin fibroblasts of healthy donors using a nonviral and nonintegrating procedure based on mRNA transfer.
In vitro transcribed mRNA for 5 factors, oct-4, nanog, klf-4, c-myc, sox-2 as well as for one new factor, hTERT, was used to induce pluripotency.
Reprogramming was analyzed by qPCR analysis of pluripotency gene expression, differentiation, gene expression array, and teratoma assays.
iPS cells were shown to express pluripotency markers and were able to differentiate towards ecto-, endo-, and mesodermal lineages.
This method may represent a safer technology for reprogramming and derivation of iPS cells.
Cells produced by this method can more easily be transferred into the clinical setting.
American Psychological Association (APA)
Arnold, Antje& Naaldijk, Yahaira M.& Fabian, Claire& Wirth, Henry& Binder, Hans& Nikkhah, Guido…[et al.]. 2011. Reprogramming of Human Huntington Fibroblasts Using mRNA. ISRN Cell Biology،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-447543
Modern Language Association (MLA)
Arnold, Antje…[et al.]. Reprogramming of Human Huntington Fibroblasts Using mRNA. ISRN Cell Biology No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-447543
American Medical Association (AMA)
Arnold, Antje& Naaldijk, Yahaira M.& Fabian, Claire& Wirth, Henry& Binder, Hans& Nikkhah, Guido…[et al.]. Reprogramming of Human Huntington Fibroblasts Using mRNA. ISRN Cell Biology. 2011. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-447543
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447543