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Cytochrome P450 102A2 Catalyzes Efficient Oxidation of Sodium Dodecyl Sulphate : A Molecular Tool for Remediation
Joint Authors
Axarli, Irene
Labrou, Nikolaos E.
Prigipaki, Ariadne
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Bacterial cytochrome P450s (CYPs) constitute an important family of monooxygenase enzymes that carry out essential roles in the metabolism of endogenous compounds and foreign chemicals.
In the present work we report the characterization of CYP102A2 from B.
subtilis with a focus on its substrate specificity.
CYP102A2 is more active in oxidation of sodium dodecyl sulphate (SDS) than any other characterized CYP.
The effect of SDS and NADPH concentration on reaction rate showed nonhyperbolic and hyperbolic dependence, respectively.
The enzyme was found to exhibit a bell-shaped curve for plots of activity versus pH, over pH values 5.9–8.5.
The rate of SDS oxidation reached the maximum value approximately at pH 7.2 and the pH transition observed controlled by two pKas in the acidic (pKa=6.7±0.08) and basic (pKa=7.3±0.06) pH range.
The results are discussed in relation to the future biotechnology applications of CYPs.
American Psychological Association (APA)
Axarli, Irene& Prigipaki, Ariadne& Labrou, Nikolaos E.. 2010. Cytochrome P450 102A2 Catalyzes Efficient Oxidation of Sodium Dodecyl Sulphate : A Molecular Tool for Remediation. Enzyme Research،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-447610
Modern Language Association (MLA)
Axarli, Irene…[et al.]. Cytochrome P450 102A2 Catalyzes Efficient Oxidation of Sodium Dodecyl Sulphate : A Molecular Tool for Remediation. Enzyme Research No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-447610
American Medical Association (AMA)
Axarli, Irene& Prigipaki, Ariadne& Labrou, Nikolaos E.. Cytochrome P450 102A2 Catalyzes Efficient Oxidation of Sodium Dodecyl Sulphate : A Molecular Tool for Remediation. Enzyme Research. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-447610
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447610