Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRB
Joint Authors
Ren, Yan
Chen, Jin-Lian
Yan, Liu-Shui
Xiong, Jing-Jing
Hu, Jun-Duo
Guo, Hui-Qin
Fan, Jing
Source
Gastroenterology Research and Practice
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Aim.
The study was to investigate the metabolic profile of urine metabolites and to elucidate their clinical significance in patients with colorectal cancer.
Methods.
Colorectal cancers from early stage and advanced stage were used in this study.
Urine samples of colorectal cancer patients and healthy adults were collected and subjected to capillary electrophoresis mass spectrometry based on moving reaction boundary analysis.
The metabolic data were analyzed by SPSS 17.0 to find urinary biomarkers for colorectal cancer.
Results.
The results indicated that the urine metabolic profiling of colorectal cancer patients had significant changes compared with the normal controls, and there were also differences between early stage and advanced colorectal cancer patients.
Compared with the control group, the levels of isoleucine, valine, arginine, lactate acid and leucine increased (P<0.05), but those of histidine, methionine, serine, aspartic acid, citric acid, succinate, and malic acid decreased in urine samples from colorectal cancer (P<0.05).
Furthermore, the levels of isoleucine and valine were lower in urine of patients with advanced colorectal cancer than those in early stage colorectal cancer (P<0.05).
Conclusion.
The technique of capillary electrophoresis mass spectrometry based on MRB could reveal the significant metabolic alterations during progression of colorectal cancer, and the method is feasible and may be useful for the early diagnosis of colorectal cancer.
American Psychological Association (APA)
Chen, Jin-Lian& Fan, Jing& Yan, Liu-Shui& Guo, Hui-Qin& Xiong, Jing-Jing& Ren, Yan…[et al.]. 2012. Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRB. Gastroenterology Research and Practice،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-447655
Modern Language Association (MLA)
Chen, Jin-Lian…[et al.]. Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRB. Gastroenterology Research and Practice No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-447655
American Medical Association (AMA)
Chen, Jin-Lian& Fan, Jing& Yan, Liu-Shui& Guo, Hui-Qin& Xiong, Jing-Jing& Ren, Yan…[et al.]. Urine Metabolite Profiling of Human Colorectal Cancer by Capillary Electrophoresis Mass Spectrometry Based on MRB. Gastroenterology Research and Practice. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-447655
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447655