![](/images/graphics-bg.png)
Establishment of OPG Transgenic Mice and the Effect of OPG on Bone Microarchitecture
Joint Authors
Liao, Eryuan
Zu, Xuyu
Liu, Jianghua
Guo, Hui
Wu, Ying
Luo, Qiong
Yu, Ziying
Source
International Journal of Endocrinology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Osteoprotegerin (OPG) plays a determinant role in regulating bone metabolism, but the effect of OPG on bone microarchitecture needs to be further elucidated.
We attempted to construct pCI-hOPGp-mOPG vector containing human OPG promoter and FLAG tag and to microinject vector into fertilized zygotes from C57BL/6J × CBA mice to prepare transgenic mice.
The OPG transgenic positive mice were identified by PCR and western blotting.
Twelve-week-old OPG transgenic mice (OPG-Tg mice) and wild-type mice (WT mice) were utilized in the study of bone microarchitecture.
Microcomputed tomography (micro-CT) data showed that compared with WT mice, the tibia of OPG-Tg mice showed an increased volumetric BMD (vBMD), tissue BMD (tBMD), trabecular thickness (Tb.Th), and trabecular number (Tb.N), and a decreased trabecular separation (Th.Sp) (P<0.05) .
The cortical bone microarchitecture parameters, such as cortical area (Ct.Ar), cortical thickness (Ct.Th), cortical BMD (Ct.BMD), cortical BMC (Ct.BMC), BMD, and BMC of femur, were increased, and the inner perimeter (In.Pm) was decreased, in OPG-Tg mice, compared to those in WT mice (P<0.05).
The established OPG transgenic mouse model could be valuable for further studying the biological significance and gene regulation of OPG in vivo.
American Psychological Association (APA)
Wu, Ying& Liu, Jianghua& Guo, Hui& Luo, Qiong& Yu, Ziying& Liao, Eryuan…[et al.]. 2013. Establishment of OPG Transgenic Mice and the Effect of OPG on Bone Microarchitecture. International Journal of Endocrinology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-447660
Modern Language Association (MLA)
Wu, Ying…[et al.]. Establishment of OPG Transgenic Mice and the Effect of OPG on Bone Microarchitecture. International Journal of Endocrinology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-447660
American Medical Association (AMA)
Wu, Ying& Liu, Jianghua& Guo, Hui& Luo, Qiong& Yu, Ziying& Liao, Eryuan…[et al.]. Establishment of OPG Transgenic Mice and the Effect of OPG on Bone Microarchitecture. International Journal of Endocrinology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-447660
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447660