A Novel Vaccine Using Nanoparticle Platform to Present Immunogenic M2e against Avian Influenza Infection
Joint Authors
Burkhard, Peter
Babapoor, Sankhiros
Neef, Tobias
Garmendia, Antonio
Shang, Hongwei
Girshick, Theodore
Mittelholzer, Christian
Khan, Mazhar I.
Source
Influenza Research and Treatment
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-12
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Using peptide nanoparticle technology, we have designed two novel vaccine constructs representing M2e in monomeric (Mono-M2e) and tetrameric (Tetra-M2e) forms.
Groups of specific pathogen free (SPF) chickens were immunized intramuscularly with Mono-M2e or Tetra-M2e with and without an adjuvant.
Two weeks after the second boost, chickens were challenged with 107.2 EID50 of H5N2 low pathogenicity avian influenza (LPAI) virus.
M2e-specific antibody responses to each of the vaccine constructs were tested by ELISA.
Vaccinated chickens exhibited increased M2e-specific IgG responses for each of the constructs as compared to a non-vaccinated group.
However, the vaccine construct Tetra-M2e elicited a significantly higher antibody response when it was used with an adjuvant.
On the other hand, virus neutralization assays indicated that immune protection is not by way of neutralizing antibodies.
The level of protection was evaluated using quantitative real time PCR at 4, 6, and 8 days post-challenge with H5N2 LPAI by measuring virus shedding from trachea and cloaca.
The Tetra-M2e with adjuvant offered statistically significant (P<0.05) protection against subtype H5N2 LPAI by reduction of the AI virus shedding.
The results suggest that the self-assembling polypeptide nanoparticle shows promise as a potential platform for a development of a vaccine against AI.
American Psychological Association (APA)
Babapoor, Sankhiros& Neef, Tobias& Mittelholzer, Christian& Girshick, Theodore& Garmendia, Antonio& Shang, Hongwei…[et al.]. 2012. A Novel Vaccine Using Nanoparticle Platform to Present Immunogenic M2e against Avian Influenza Infection. Influenza Research and Treatment،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-447731
Modern Language Association (MLA)
Babapoor, Sankhiros…[et al.]. A Novel Vaccine Using Nanoparticle Platform to Present Immunogenic M2e against Avian Influenza Infection. Influenza Research and Treatment No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-447731
American Medical Association (AMA)
Babapoor, Sankhiros& Neef, Tobias& Mittelholzer, Christian& Girshick, Theodore& Garmendia, Antonio& Shang, Hongwei…[et al.]. A Novel Vaccine Using Nanoparticle Platform to Present Immunogenic M2e against Avian Influenza Infection. Influenza Research and Treatment. 2012. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-447731
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447731