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Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus
Joint Authors
Zhao, Jing-jing
Chen, Chang
Hu, Chao-qun
Zhang, Lv-ping
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Vibrio alginolyticus ZJ-51 displays phase variation between opaque/rugose colonies (Op) and translucent/smooth colonies (Tr).
These colony variants show great differences in biofilm formation and motility.
In this study, a gene encoding for an rpoS-like sigma factor, rpoX, has been cloned and characterized.
The absence of rpoX did not affect colony switching rate but did decrease biofilm formation in both the Op and the Tr variants.
When challenged with hydrogen peroxide, the ΔrpoX in the Op background showed a slightly higher survival rate compared with the wild type, whereas survival was decreased in the Tr background.
Deletion of rpoX in the Tr background resulted in a higher ability to resist ethanol challenges and to survive hyperosmolarity challenges, and in the Op background the opposite phenotype was observed.
This indicates that the rpoX gene is involved in biofilm formation and stress response but the effects are controlled by colony phase variation in V.
alginolyticus.
American Psychological Association (APA)
Zhao, Jing-jing& Chen, Chang& Zhang, Lv-ping& Hu, Chao-qun. 2009. Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-447748
Modern Language Association (MLA)
Zhao, Jing-jing…[et al.]. Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-447748
American Medical Association (AMA)
Zhao, Jing-jing& Chen, Chang& Zhang, Lv-ping& Hu, Chao-qun. Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-447748
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447748