Expression and Function of Ephrin-B1 and Its Cognate Receptor EphB2 in Human Abdominal Aortic Aneurysm
Joint Authors
Sugamoto, Yuka
Konno, Tetsuo
Yoshimuta, Tsuyoshi
Sakamoto, Aiji
Kawashiri, Masaaki
Hayashi, Kenshi
Ogino, Hitoshi
Ishibashi-Ueda, Hatsue
Higashikata, Takeo
Yamagishi, Masakazu
Tada, Hayato
Source
International Journal of Vascular Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We examined the expression of ephrin-B1 and its cognate receptor EphB2, key regulators of angiogenesis and embryogenesis, in human abdominal aortic aneurysm (AAA) and analyzed their functional roles in cell migration.
From 10 patients (9 males and 1 female; age, 68.5±2.4) who underwent vascular surgery for AAA, we obtained AAA and adjacent control tissues.
Using real-time RT-PCR, we analyzed expression of ephrin-B1 and EphB2.
We also histologically localized these molecules in AAA tissues.
Finally, effects of ephrin-B1 and EphB2 on inflammatory cell chemotaxis were examined by cell migration assay.
Expression levels of ephrin-B1 (0.410±0.046 versus 1.198±0.252, P=0.027) and EphB2 (0.764±0.212 versus 1.272±0.137, P=0.594) were higher in AAA than normal control.
Both ephrin-B1 and EphB2 were expressed in macrophages, T lymphocytes, and endothelial cells within AAA.
In chemotaxis assay, ephrin-B1 and EphB2 inhibited mononuclear-cell chemotaxis induced by stromal derived factor-1 down to 54.7±12.7% (P=0.01) and 50.7±13.1% (P=0.01), respectively.
These data suggest that ephrin-B1 and EphB2 might be functional in human adult inflammatory cells and involved in the pathogenesis of AAA, although specific roles of these molecules should further be sought.
American Psychological Association (APA)
Sakamoto, Aiji& Kawashiri, Masaaki& Ishibashi-Ueda, Hatsue& Sugamoto, Yuka& Yoshimuta, Tsuyoshi& Higashikata, Takeo…[et al.]. 2012. Expression and Function of Ephrin-B1 and Its Cognate Receptor EphB2 in Human Abdominal Aortic Aneurysm. International Journal of Vascular Medicine،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-447768
Modern Language Association (MLA)
Sakamoto, Aiji…[et al.]. Expression and Function of Ephrin-B1 and Its Cognate Receptor EphB2 in Human Abdominal Aortic Aneurysm. International Journal of Vascular Medicine No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-447768
American Medical Association (AMA)
Sakamoto, Aiji& Kawashiri, Masaaki& Ishibashi-Ueda, Hatsue& Sugamoto, Yuka& Yoshimuta, Tsuyoshi& Higashikata, Takeo…[et al.]. Expression and Function of Ephrin-B1 and Its Cognate Receptor EphB2 in Human Abdominal Aortic Aneurysm. International Journal of Vascular Medicine. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-447768
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447768