Dual Specificity Phosphatase 1 Regulates Human Inducible Nitric Oxide Synthase Expression by p38 MAP Kinase
Joint Authors
Taimi, Ville
Korhonen, Riku
Moilanen, Eeva
Turpeinen, Tuija
Heittola, Taina
Clark, Andrew R.
Sareila, Outi
Nieminen, Riina
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-19
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The role of dual specificity phosphatase 1 (DUSP1) in inducible nitric oxide synthase (iNOS) expression in A549 human pulmonary epithelial cells, J774 mouse macrophages and primary mouse bone marrow-derived macrophages (BMMs) was investigated.
iNOS expression was induced by a cytokine mixture (TNF, IFNγ and IL-1β) in A549 cells and by LPS in J774 cells, and it was inhibited by p38 MAPK inhibitors SB202190 and BIRB 796.
Stimulation with cytokine mixture or LPS enhanced also DUSP1 expression.
Down-regulation of DUSP1 by siRNA increased p38 MAPK phosphorylation and iNOS expression in A549 and J774 cells.
In addition, LPS-induced iNOS expression was enhanced in BMMs from DUSP1(−/−) mice as compared to that in BMMs from wild-type mice.
The results indicate that DUSP1 suppresses iNOS expression by limiting p38 MAPK activity in human and mouse cells.
Compounds that enhance DUSP1 expression or modulate its function may be beneficial in diseases complicated with increased iNOS-mediated NO production.
American Psychological Association (APA)
Turpeinen, Tuija& Nieminen, Riina& Taimi, Ville& Heittola, Taina& Sareila, Outi& Clark, Andrew R.…[et al.]. 2011. Dual Specificity Phosphatase 1 Regulates Human Inducible Nitric Oxide Synthase Expression by p38 MAP Kinase. Mediators of Inflammation،Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-447820
Modern Language Association (MLA)
Turpeinen, Tuija…[et al.]. Dual Specificity Phosphatase 1 Regulates Human Inducible Nitric Oxide Synthase Expression by p38 MAP Kinase. Mediators of Inflammation No. 2011 (2011), pp.1-15.
https://search.emarefa.net/detail/BIM-447820
American Medical Association (AMA)
Turpeinen, Tuija& Nieminen, Riina& Taimi, Ville& Heittola, Taina& Sareila, Outi& Clark, Andrew R.…[et al.]. Dual Specificity Phosphatase 1 Regulates Human Inducible Nitric Oxide Synthase Expression by p38 MAP Kinase. Mediators of Inflammation. 2011. Vol. 2011, no. 2011, pp.1-15.
https://search.emarefa.net/detail/BIM-447820
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447820