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Molecular Mechanisms Underlying the In Vitro Anti-Inflammatory Effects of a Flavonoid-Rich Ethanol Extract from Chinese Propolis (Poplar Type)
Joint Authors
Wang, Kai
Hu, Lin
Xuan, Hongzhuan
Huang, Shuai
Ping, Shun
Zhang, Cuiping
Hu, Fuliang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
China produces the greatest amount of propolis but there is still lack of basic studies on its pharmacological mechanisms.
Our previous study found that ethanol extract from Chinese propolis (EECP) exerted excellent anti-inflammatory effects in vivo but mechanisms of action were elusive.
To further clarify the possible mechanisms underlying the anti-inflammatory effects of Chinese propolis (poplar type), we utilized EECP to analyze its chemical composition and evaluated its potential anti-inflammatory effects in vitro.
High-performance liquid chromatography (HPLC) profile indicated that EECP contained abundant flavonoids, including rutin, myricetin, quercetin, kaempferol, apigenin, pinocembrin, chrysin, and galangin.
Next we found that EECP could significantly inhibit the production of NO, IL-1β, and IL-6 in lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells and suppress mRNA expression of iNOS, IL-1β, and IL-6 in a time- and dose-dependent manner.
Furthermore, we found that EECP could suppress the phosphorylation of IκBα and AP-1 but did not affect IκBα’s degradation.
In addition, using a reporter assay, we found that EECP could block the activation of NF-κB in TNF-α-stimulated HEK 293T cells.
Our findings give new insights for understanding the mechanisms involved in the anti-inflammatory effects by Chinese propolis and provide additional references for using propolis in alternative and complementary therapies.
American Psychological Association (APA)
Wang, Kai& Ping, Shun& Huang, Shuai& Hu, Lin& Xuan, Hongzhuan& Zhang, Cuiping…[et al.]. 2013. Molecular Mechanisms Underlying the In Vitro Anti-Inflammatory Effects of a Flavonoid-Rich Ethanol Extract from Chinese Propolis (Poplar Type). Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-447830
Modern Language Association (MLA)
Wang, Kai…[et al.]. Molecular Mechanisms Underlying the In Vitro Anti-Inflammatory Effects of a Flavonoid-Rich Ethanol Extract from Chinese Propolis (Poplar Type). Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-447830
American Medical Association (AMA)
Wang, Kai& Ping, Shun& Huang, Shuai& Hu, Lin& Xuan, Hongzhuan& Zhang, Cuiping…[et al.]. Molecular Mechanisms Underlying the In Vitro Anti-Inflammatory Effects of a Flavonoid-Rich Ethanol Extract from Chinese Propolis (Poplar Type). Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-447830
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447830