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Biodegradation Ability and Catabolic Genes of Petroleum-Degrading Sphingomonas koreensis Strain ASU-06 Isolated from Egyptian Oily Soil
Joint Authors
Hesham, Abd El-Latif
Shoreit, Ahmed
Mawad, Asmaa M. M.
Mustafa, Yasser Mohamed
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Polycyclic aromatic hydrocarbons (PAHs) are serious pollutants and health hazards.
In this study, 15 PAHs-degrading bacteria were isolated from Egyptian oily soil.
Among them, one Gram-negative strain (ASU-06) was selected and biodegradation ability and initial catabolic genes of petroleum compounds were investigated.
Comparison of 16S rRNA gene sequence of strain ASU-06 to published sequences in GenBank database as well as phylogenetic analysis identified ASU-06 as Sphingomonas koreensis.
Strain ASU-06 degraded 100, 99, 98, and 92.7% of 100 mg/L naphthalene, phenanthrene, anthracene, and pyrene within 15 days, respectively.
When these PAHs present in a mixed form, the enhancement phenomenon appeared, particularly in the degradation of pyrene, whereas the degradation rate was 98.6% within the period.
This is the first report showing the degradation of different PAHs by this species.
PCR experiments with specific primers for catabolic genes alkB, alkB1, nahAc, C12O, and C23O suggested that ASU-06 might possess genes for aliphatic and PAHs degradation, while PAH-RHDαGP gene was not detected.
Production of biosurfactants and increasing cell-surface hydrophobicity were investigated.
GC/MS analysis of intermediate metabolites of studied PAHs concluded that this strain utilized these compounds via two main pathways, and phthalate was the major constant product that appeared in each day of the degradation period.
American Psychological Association (APA)
Hesham, Abd El-Latif& Mawad, Asmaa M. M.& Mustafa, Yasser Mohamed& Shoreit, Ahmed. 2014. Biodegradation Ability and Catabolic Genes of Petroleum-Degrading Sphingomonas koreensis Strain ASU-06 Isolated from Egyptian Oily Soil. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-447831
Modern Language Association (MLA)
Hesham, Abd El-Latif…[et al.]. Biodegradation Ability and Catabolic Genes of Petroleum-Degrading Sphingomonas koreensis Strain ASU-06 Isolated from Egyptian Oily Soil. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-447831
American Medical Association (AMA)
Hesham, Abd El-Latif& Mawad, Asmaa M. M.& Mustafa, Yasser Mohamed& Shoreit, Ahmed. Biodegradation Ability and Catabolic Genes of Petroleum-Degrading Sphingomonas koreensis Strain ASU-06 Isolated from Egyptian Oily Soil. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-447831
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447831