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Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin
Joint Authors
Dallo, Shatha F.
Ye, Jing Yong
Hong, Soonbae
Tsai, Anyu
Zhang, Bailin
Weitao, Tao
Denno, James
Haskins, William E.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
A conundrum has long lingered over association of cytosol elongation factor Tu (EF-Tu) with bacterial surface.
Here we investigated it with Acinetobacter baumannii, an emerging opportunistic pathogen associated with a wide spectrum of infectious diseases.
The gene for A.
baumannii EF-Tu was sequenced, and recombinant EF-Tu was purified for antibody development.
EF-Tu on the bacterial surface and the outer membrane vesicles (OMVs) was revealed by immune electron microscopy, and its presence in the outer membrane (OM) and the OMV subproteomes was verified by Western blotting with the EF-Tu antibodies and confirmed by proteomic analyses.
EF-Tu in the OM and the OMV subproteomes bound to fibronectin as detected by Western blot and confirmed by a label-free real-time optical sensor.
The sensor that originates from photonic crystal structure in a total-Internal-reflection (PC-TIR) configuration was functionalized with fibronectin for characterizing EF-Tu binding.
Altogether, with a novel combination of immunological, proteomical, and biophysical assays, these results suggest association of A.
baumannii EF-Tu with the bacterial cell surface, OMVs, and fibronectin.
American Psychological Association (APA)
Dallo, Shatha F.& Zhang, Bailin& Denno, James& Hong, Soonbae& Tsai, Anyu& Haskins, William E.…[et al.]. 2012. Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-447934
Modern Language Association (MLA)
Dallo, Shatha F.…[et al.]. Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin. The Scientific World Journal No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-447934
American Medical Association (AMA)
Dallo, Shatha F.& Zhang, Bailin& Denno, James& Hong, Soonbae& Tsai, Anyu& Haskins, William E.…[et al.]. Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-447934
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447934