Adipose Tissue-Derived Stem Cell Secreted IGF-1 Protects Myoblasts from the Negative Effect of Myostatin
Joint Authors
Krutsch, Werner
Gosau, Martin
Klein, Silvan
Schreml, Stephan
Gehmert, Sebastian
Wenzel, Carina
Prantl, Lukas
Huber, Michaela
Nerlich, Michael
Gehmert, Sanga
Brockhoff, Gero
Loibl, Markus
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Myostatin, a TGF-β family member, is associated with inhibition of muscle growth and differentiation and might interact with the IGF-1 signaling pathway.
Since IGF-1 is secreted at a bioactive level by adipose tissue-derived mesenchymal stem cells (ASCs), these cells (ASCs) provide a therapeutic option for Duchenne Muscular Dystrophy (DMD).
But the protective effect of stem cell secreted IGF-1 on myoblast under high level of myostatin remains unclear.
In the present study murine myoblasts were exposed to myostatin under presence of ASCs conditioned medium and investigated for proliferation and apoptosis.
The protective effect of IGF-1 was further examined by using IGF-1 neutralizing and receptor antibodies as well as gene silencing RNAi technology.
MyoD expression was detected to identify impact of IGF-1 on myoblasts differentiation when exposed to myostatin.
IGF-1 was accountable for 43.6% of the antiapoptotic impact and 48.8% for the proliferative effect of ASCs conditioned medium.
Furthermore, IGF-1 restored mRNA and protein MyoD expression of myoblasts under risk.
Beside fusion and transdifferentiation the beneficial effect of ASCs is mediated by paracrine secreted cytokines, particularly IGF-1.
The present study underlines the potential of ASCs as a therapeutic option for Duchenne muscular dystrophy and other dystrophic muscle diseases.
American Psychological Association (APA)
Gehmert, Sebastian& Wenzel, Carina& Loibl, Markus& Brockhoff, Gero& Huber, Michaela& Krutsch, Werner…[et al.]. 2014. Adipose Tissue-Derived Stem Cell Secreted IGF-1 Protects Myoblasts from the Negative Effect of Myostatin. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-447949
Modern Language Association (MLA)
Gehmert, Sebastian…[et al.]. Adipose Tissue-Derived Stem Cell Secreted IGF-1 Protects Myoblasts from the Negative Effect of Myostatin. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-447949
American Medical Association (AMA)
Gehmert, Sebastian& Wenzel, Carina& Loibl, Markus& Brockhoff, Gero& Huber, Michaela& Krutsch, Werner…[et al.]. Adipose Tissue-Derived Stem Cell Secreted IGF-1 Protects Myoblasts from the Negative Effect of Myostatin. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-447949
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447949