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Prediction of Spontaneous Regression of Cervical Intraepithelial Neoplasia Lesions Grades 2 and 3 by Proteomic Analysis
Joint Authors
Janssen, Emiel A. M.
Diermen, Bianca van
Øvestad, Irene Tveiterås
Uleberg, Kai-Erik
Baak, Jan P. A.
Munk, Ane Cecilie
Brede, Cato
Gudlaugsson, Einar
Hjelle, Anne
Source
International Journal of Proteomics
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-15
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Topics
Abstract EN
Regression of cervical intraepithelial neoplasia (CIN) 2-3 to CIN 1 or less is associated with immune response as demonstrated by immunohistochemistry in formaldehyde-fixed paraffin-embedded (FFPE) biopsies.
Proteomic analysis of water-soluble proteins in supernatants of biopsy samples with LC-MS (LTQ-Orbitrap) was used to identify proteins predictive of CIN2-3 lesions regression.
CIN2-3 in the biopsies and persistence (CIN2-3) or regression (≤CIN1) in follow-up cone biopsies was validated histologically by two experienced pathologists.
In a learning set of 20 CIN2-3 (10 regressions and 10 persistence cases), supernatants were depleted of seven high abundance proteins prior to unidimensional LC-MS/MS protein analysis.
Mean protein concentration was 0.81 mg/mL (range: 0.55–1.14).
Multivariate statistical methods were used to identify proteins that were able to discriminate between regressive and persistent CIN2-3.
The findings were validated in an independent test set of 20 CIN2-3 (10 regressions and 10 persistence cases).
Multistep identification criteria identified 165 proteins.
In the learning set, zinc finger protein 441 and phospholipase D6 independently discriminated between regressive and persistent CIN2-3 lesions and correctly classified all 20 patients.
Nine regression and all persistence cases were correctly classified in the validation set.
Zinc finger protein 441 and phospholipase D6 in supernatant samples detected by LTQ-Orbitrap can predict regression of CIN2-3.
American Psychological Association (APA)
Uleberg, Kai-Erik& Øvestad, Irene Tveiterås& Munk, Ane Cecilie& Brede, Cato& Diermen, Bianca van& Gudlaugsson, Einar…[et al.]. 2014. Prediction of Spontaneous Regression of Cervical Intraepithelial Neoplasia Lesions Grades 2 and 3 by Proteomic Analysis. International Journal of Proteomics،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-447950
Modern Language Association (MLA)
Uleberg, Kai-Erik…[et al.]. Prediction of Spontaneous Regression of Cervical Intraepithelial Neoplasia Lesions Grades 2 and 3 by Proteomic Analysis. International Journal of Proteomics No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-447950
American Medical Association (AMA)
Uleberg, Kai-Erik& Øvestad, Irene Tveiterås& Munk, Ane Cecilie& Brede, Cato& Diermen, Bianca van& Gudlaugsson, Einar…[et al.]. Prediction of Spontaneous Regression of Cervical Intraepithelial Neoplasia Lesions Grades 2 and 3 by Proteomic Analysis. International Journal of Proteomics. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-447950
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447950