Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products
Joint Authors
Muhr, Alexander
Hermann-Krauss, Carmen
Koller, Martin
Braunegg, Gerhart
Fasl, Hubert
Stelzer, Franz
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The archaeon Haloferax mediterranei was selected for production of PHA co- and terpolyesters using inexpensive crude glycerol phase (CGP) from biodiesel production as carbon source.
CGP was assessed by comparison with the application of pure glycerol.
Applying pure glycerol, a copolyester with a molar fraction of 3-hydroxybutyrate (3HB) of 0.90 mol/mol and 3-hydroxyvalerate (3HV) of 0.10 mol/mol, was produced at a volumetric productivity of 0.12 g/Lh and an intracellular PHA content of 75.4 wt.-% in the sum of biomass protein plus PHA.
Application of CGP resulted in the same polyester composition and volumetric productivity, indicating the feasibility of applying CGP as feedstock.
Analysis of molar mass distribution revealed a weight average molar mass Mw of 150 kDa and polydispersity Pi of 2.1 for pure glycerol and 253 kDa and 2.7 for CGP, respectively; melting temperatures ranged between 130 and 140°C in both setups.
Supplying γ-butyrolactone as 4-hydroxybutyrate (4HB) precursor resulted in a poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate-co-4-hydroxybutyrate] (PHBHV4HB) terpolyester containing 3HV (0.12 mol/mol) and 4HB (0.05 mol/mol) in the poly[(R)-3-hydroxybutyrate] (PHB) matrix; in addition, this process runs without sterilization of the bioreactor.
The terpolyester displayed reduced melting (melting endotherms at 122 and 137°C) and glass transition temperature (2.5°C), increased molar mass (391 kDa), and a polydispersity similar to the copolyesters.
American Psychological Association (APA)
Hermann-Krauss, Carmen& Koller, Martin& Muhr, Alexander& Fasl, Hubert& Stelzer, Franz& Braunegg, Gerhart. 2013. Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products. Archaea،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-447973
Modern Language Association (MLA)
Hermann-Krauss, Carmen…[et al.]. Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products. Archaea No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-447973
American Medical Association (AMA)
Hermann-Krauss, Carmen& Koller, Martin& Muhr, Alexander& Fasl, Hubert& Stelzer, Franz& Braunegg, Gerhart. Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products. Archaea. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-447973
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447973