Immobilized Horseradish Peroxidase on Discs of Polyvinyl Alcohol-Glutaraldehyde Coated with Polyaniline
Joint Authors
Caramori, Samantha Salomão
de Carvalho Junior, Luiz Bezerra
Fernandes, Kátia Flávia
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Discs of network polyvinyl alcohol-glutaraldehyde (PVAG) were synthesized and coated with polyaniline (PANI) using glutaraldehyde as a chemical arm (PVAG-PANIG-HRP disc).
The best conditions for the immobilization were established as about 1.0 mg mL−1 of protein, for 60 min and pH 5.5.
The soluble enzyme lost all of its activity after incubation at 70°C for 15 min, whereas the PVAG-PANIG-HRP disc retained about half of the initial activity for pyrogallol.
The same PVAG-PANIG-HRP disc was used consecutively three times without any activity lossbut presented 25% of the initial activity after the 7th use.
PVAG-PANIG-HRP disc retained approximately 80% and 60% of its initial activity after 60 and 80 days of storage, respectively.
Resorcinol, m-cresol, catechol, pyrogallol, α-naphthol, βnaphthol, and 4, 4′-diaminodiphenyl benzidine were efficiently oxidized by the PVAG-PANIG-HRP disc (from about 70% to 90%), and it was less efficient towards aniline, phenol, and 2-nitrosonaphthol.
American Psychological Association (APA)
Caramori, Samantha Salomão& Fernandes, Kátia Flávia& de Carvalho Junior, Luiz Bezerra. 2012. Immobilized Horseradish Peroxidase on Discs of Polyvinyl Alcohol-Glutaraldehyde Coated with Polyaniline. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-448027
Modern Language Association (MLA)
Caramori, Samantha Salomão…[et al.]. Immobilized Horseradish Peroxidase on Discs of Polyvinyl Alcohol-Glutaraldehyde Coated with Polyaniline. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-448027
American Medical Association (AMA)
Caramori, Samantha Salomão& Fernandes, Kátia Flávia& de Carvalho Junior, Luiz Bezerra. Immobilized Horseradish Peroxidase on Discs of Polyvinyl Alcohol-Glutaraldehyde Coated with Polyaniline. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-448027
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448027